ArticleProceedings of the National Academy of Sciences of the United States of America2014
Bloom of resident antibiotic-resistant bacteria in soil following manure fertilization.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 135 papers, 1 of them a synthesis that pooled it.
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Who cites it
135 citing papers in PubMed, 1 synthesis or guideline pooled it, 581 citations in OpenAlex.
- One Health and Global Health View of Antimicrobial Susceptibility through the "Eye" of Aeromonas: Systematic Review and Meta-Analysis.International journal of antimicrobial agents · 2023Pooled it
- Article
- Dairy manure, glyphosate, and antimicrobials (copper, streptomycin, and triazole) modulated the composition of antimicrobial resistance at the gene and microbial levels in a processing tomato field.Microbiology spectrum · 2026Article
- Impacts of drought and manure fertilization on soil and radish resistomes.Scientific reports · 2026Article
- Occurrence and antimicrobial resistance profiles of bacteria from dairy farm environments in Addis Ababa, Ethiopia.BMC microbiology · 2026Article
- Antimicrobial resistance in plant-associated microbial communities: mechanisms, ecology, and one health implications.Frontiers in microbiology · 2026Review
- Evaluation of plant-based antimicrobials in controlling antimicrobial resistance gene spread in poultry production continuum.Frontiers in veterinary science · 2026Article
- Antimicrobial resistance within the one health lens: global drivers, mechanisms and public health policy gaps in low resource settings.Frontiers in cellular and infection microbiology · 2026Review
- Exploring the Relationship Between Farmland Management and Manure-Derived Antibiotic Resistance Genes and Their Prevention and Control Strategies.Antibiotics (Basel, Switzerland) · 2025Review
- Harnessing phage consortia to mitigate the soil antibiotic resistome by targeting keystone taxa Streptomyces.Microbiome · 2025Article
- Environmental fate of antibiotic resistance genes in livestock farming.Archives of microbiology · 2025Review
- Manure-Amended One-Year-Reclamation Promoted Soil Bacterial Phylotypic and Phenotypic Shifts in a Typical Coal-Mining Area.Microorganisms · 2025Article
- Microbial interactions between climate warming and antimicrobial resistance threaten soil carbon storage and global health.The ISME journal · 2025Article
- The gut microbiome: an emerging epicenter of antimicrobial resistance?Frontiers in microbiology · 2025Review
- Harnessing beneficial bacteria to remediate antibiotic-polluted agricultural soils: integrating source diversity, bioavailability modulators, and ecological impacts.Frontiers in microbiology · 2025Review
- Comparative analysis of antibiotic resistance genes between fresh pig manure and composted pig manure in winter, China.PloS one · 2025Article
- Threats across boundaries: the spread of ESBL-positiveFrontiers in microbiology · 2025Review
- Temporal signals in dairy cattle slurry and fertilized field soil resistomes and bacterial communities.Frontiers in microbiology · 2025Article
- The soil-microbe-plant resistome: A focus on the source-pathway-receptor continuum.Environmental science and pollution research international · 2024Review
- The fly route of extended-spectrum-β-lactamase-producingFrontiers in antibiotics · 2024Article
75 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
The increasing prevalence of antibiotic-resistant bacteria is a global threat to public health. Agricultural use of antibiotics is believed to contribute to the spread of antibiotic resistance, but the mechanisms by which many agricultural practices influence resistance remain obscure. Although manure from dairy farms is a common soil amendment in crop production, its impact on the soil microbiome and resistome is not known. To gain insight into this impact, we cultured bacteria from soil before and at 10 time points after application of manure from cows that had not received antibiotic treatment. Soil treated with manure contained a higher abundance of β-lactam-resistant bacteria than soil treated with inorganic fertilizer. Functional metagenomics identified β-lactam-resistance genes in treated and untreated soil, and indicated that the higher frequency of resistant bacteria in manure-amended soil was attributable to enrichment of resident soil bacteria that harbor β-lactamases. Quantitative PCR indicated that manure treatment enriched the blaCEP-04 gene, which is highly similar (96%) to a gene found previously in a Pseudomonas sp. Analysis of 16S rRNA genes indicated that the abundance of Pseudomonas spp. increased in manure-amended soil. Populations of other soil bacteria that commonly harbor β-lactamases, including Janthinobacterium sp. and Psychrobacter pulmonis, also increased in response to manure treatment. These results indicate that manure amendment induced a bloom of certain antibiotic-resistant bacteria in soil that was independent of antibiotic exposure of the cows from which the manure was derived. Our data illustrate the unintended consequences that can result from agricultural practices, and demonstrate the need for empirical analysis of the agroecosystem.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.