ArticleProceedings of the National Academy of Sciences of the United States of America2014
Label-free electrochemical detection of human methyltransferase from tumors.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 75 citations in OpenAlex.
- Biosensors for Public Health and Environmental Monitoring: The Case for Sustainable Biosensing.ACS sustainable chemistry & engineering · 2024Review
- Highly Efficient Carbon Dioxide Electroreduction via DNA-Directed Catalyst Immobilization.JACS Au · 2024Article
- A quantum physics layer of epigenetics: a hypothesis deduced from charge transfer and chirality-induced spin selectivity of DNA.Clinical epigenetics · 2023Article
- Blocker-dUThiophene poly tailing-based method for assessing methyl transferase activity.Analytical and bioanalytical chemistry · 2023Article
- An Allosteric Transcription Factor DNA-Binding Electrochemical Biosensor for Progesterone.ACS sensors · 2022Article
- Emerging biosensing and transducing techniques for potential applications in point-of-care diagnostics.Chemical science · 2022Review
- DNA Electrochemistry: Charge-Transport Pathways through DNA Films on Gold.Journal of the American Chemical Society · 2021Article
- Detecting Attomolar DNA-Damaging Anticancer Drug Activity in Cell Lysates with Electrochemical DNA Devices.ACS sensors · 2021Article
- DNA Methylation in Solid Tumors: Functions and Methods of Detection.International journal of molecular sciences · 2021Review
- An Integrated Multi-Function Heterogeneous Biochemical Circuit for High-Resolution Electrochemistry-Based Genetic Analysis.Angewandte Chemie (International ed. in English) · 2020Article
- Beyond a Ribosomal RNA Methyltransferase, the Wider Role of MraW in DNA Methylation, Motility and Colonization inFrontiers in microbiology · 2019Article
- Following anticancer drug activity in cell lysates with DNA devices.Biosensors & bioelectronics · 2018Article
- Sensing DNA through DNA Charge Transport.ACS chemical biology · 2018Review
- Electrochemical affinity biosensors for fast detection of gene-specific methylations with no need for bisulfite and amplification treatments.Scientific reports · 2018Article
- Electrical Probes of DNA-Binding Proteins.Methods in enzymology · 2017Article
- DNA Charge Transport: from Chemical Principles to the Cell.Cell chemical biology · 2016Review
- DNA Methyltransferase Activity Assays: Advances and Challenges.Theranostics · 2016Review
- Reactivity of Nucleic Acid Radicals.Advances in physical organic chemistry · 2016Article
- DNA Electrochemistry Shows DNMT1 Methyltransferase Hyperactivity in Colorectal Tumors.Chemistry & biology · 2015Article
- A Multiplexed, Two-Electrode Platform for Biosensing Based on DNA-Mediated Charge Transport.Langmuir : the ACS journal of surfaces and colloids · 2015Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The role of abnormal DNA methyltransferase activity in the development and progression of cancer is an essential and rapidly growing area of research, both for improved diagnosis and treatment. However, current technologies for the assessment of methyltransferase activity, particularly from crude tumor samples, limit this work because they rely on radioactivity or fluorescence and require bulky instrumentation. Here, we report an electrochemical platform that overcomes these limitations for the label-free detection of human DNA(cytosine-5)-methyltransferase1 (DNMT1) methyltransferase activity, enabling measurements from crude cultured colorectal cancer cell lysates (HCT116) and biopsied tumor tissues. Our multiplexed detection system involving patterning and detection from a secondary electrode array combines low-density DNA monolayer patterning and electrocatalytically amplified DNA charge transport chemistry to measure selectively and sensitively DNMT1 activity within these complex and congested cellular samples. Based on differences in DNMT1 activity measured with this assay, we distinguish colorectal tumor tissue from healthy adjacent tissue, illustrating the effectiveness of this two-electrode platform for clinical applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.