ArticleProceedings of the National Academy of Sciences of the United States of America2014
Single-molecule packaging initiation in real time by a viral DNA packaging machine from bacteriophage T4.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Article
- The Fantastic Single-Molecule Techniques.Chemical & biomedical imaging · 2026Review
- Bacteriophage T4 genome packaging: mechanism and application.EcoSal Plus · 2025Review
- Virion content unpacked by long-read sequencing: stress-induced changes in transmitted staphylococcal mobilome due to phage-satellite interactions.Nucleic acids research · 2025Article
- Viral Genomic DNA Packaging Machinery.Sub-cellular biochemistry · 2024Review
- Design of bacteriophage T4-based artificial viral vectors for human genome remodeling.Nature communications · 2023Article
- Review
- Knockdown of capsid protein encoding novel ATPase domain inhibits genome packaging in potato leafroll virus.3 Biotech · 2022Article
- A viral genome packaging ring-ATPase is a flexibly coordinated pentamer.Nature communications · 2021Article
- Expression and purification of nuclease-free protocatechuate 3,4-dioxygenase for prolonged single-molecule fluorescence imaging.Analytical biochemistry · 2018Article
- Artificial bio-nanomachines based on protein needles derived from bacteriophage T4.Biophysical reviews · 2018Review
- Altering the speed of a DNA packaging motor from bacteriophage T4.Nucleic acids research · 2017Article
- Energetic cost of building a virus.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Walker-A Motif Acts to Coordinate ATP Hydrolysis with Motor Output in Viral DNA Packaging.Journal of molecular biology · 2016Article
- Next-Generation "-omics" Approaches Reveal a Massive Alteration of Host RNA Metabolism during Bacteriophage Infection of Pseudomonas aeruginosa.PLoS genetics · 2016Article
- Exclusion of small terminase mediated DNA threading models for genome packaging in bacteriophage T4.Nucleic acids research · 2016Article
- Mechanisms of DNA Packaging by Large Double-Stranded DNA Viruses.Annual review of virology · 2015Review
- Structure and mechanism of the ATPase that powers viral genome packaging.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Designing a nine cysteine-less DNA packaging motor from bacteriophage T4 reveals new insights into ATPase structure and function.Virology · 2014Article
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Authors and funding
9 authors.
Funding
Abstract
Viral DNA packaging motors are among the most powerful molecular motors known. A variety of structural, biochemical, and single-molecule biophysical approaches have been used to understand their mechanochemistry. However, packaging initiation has been difficult to analyze because of its transient and highly dynamic nature. Here, we developed a single-molecule fluorescence assay that allowed visualization of packaging initiation and reinitiation in real time and quantification of motor assembly and initiation kinetics. We observed that a single bacteriophage T4 packaging machine can package multiple DNA molecules in bursts of activity separated by long pauses, suggesting that it switches between active and quiescent states. Multiple initiation pathways were discovered including, unexpectedly, direct DNA binding to the capsid portal followed by recruitment of motor subunits. Rapid succession of ATP hydrolysis was essential for efficient initiation. These observations have implications for the evolution of icosahedral viruses and regulation of virus assembly.
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Registered trials
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