Evidence map›Paper›PMID 25288639›Full record

ArticleMolecular and cellular biology2014

Tropomyosin Tm5NM1 spatially restricts src kinase activity through perturbation of Rab11 vesicle trafficking.

Cuc T Bach, Rachael Z Murray, Dylan Owen, Kat Gaus, Geraldine M O'Neill

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular and cellular biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact, top 96% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 0 citations in OpenAlex.

  1. Article
  2. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Cuc T BachChildren's Cancer Research Unit, Kids Research Institute, The Children's Hospital at Westmead, Sydney, Australia Discipline of Paediatrics and Child Health, The University of Sydney, Sydney, Australia.
Rachael Z MurrayInstitute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Australia.
Dylan OwenCentre for Vascular Research and Australian Centre for NanoMedicine, University of New South Wales, Sydney, Australia.
Kat GausCentre for Vascular Research and Australian Centre for NanoMedicine, University of New South Wales, Sydney, Australia.
Geraldine M O'NeillChildren's Cancer Research Unit, Kids Research Institute, The Children's Hospital at Westmead, Sydney, Australia Discipline of Paediatrics and Child Health, The University of Sydney, Sydney, Australia geraldine.oneill@health.nsw.gov.au.
The University of Sydney · AUUNSW Sydney · AUQueensland University of Technology · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In order for cells to stop moving, they must synchronously stabilize actin filaments and their associated focal adhesions. How these two structures are coordinated in time and space is not known. We show here that the actin association protein Tm5NM1, which induces stable actin filaments, concurrently suppresses the trafficking of focal-adhesion-regulatory molecules. Using combinations of fluorescent biosensors and fluorescence recovery after photobleaching (FRAP), we demonstrate that Tm5NM1 reduces the level of delivery of Src kinase to focal adhesions, resulting in reduced phosphorylation of adhesion-resident Src substrates. Live imaging of Rab11-positive recycling endosomes that carry Src to focal adhesions reveals disruption of this pathway. We propose that tropomyosin synchronizes adhesion dynamics with the cytoskeleton by regulating actin-dependent trafficking of essential focal-adhesion molecules.

Indexed as

Actin CytoskeletonAnimalsCell LineEndosomesFocal AdhesionsMicePhosphorylationrab11 GTP-Binding Proteinsrab GTP-Binding ProteinsRatssrc-Family KinasesTropomyosinrab11 GTP-Binding Proteinsrab GTP-Binding Proteinssrc-Family KinasesTropomyosin

Identifiers

PMID25288639
PMCPMC4248726
OpenAlexW1523729676

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.