Evidence map›Paper›PMID 25286950›Full record

ArticleActa crystallographica. Section F, Structural biology communications2014

Crystallization and preliminary X-ray diffraction analyses of the redox-controlled complex of terminal oxygenase and ferredoxin components in the Rieske nonhaem iron oxygenase carbazole 1,9a-dioxygenase.

Jun Matsuzawa, Hiroki Aikawa, Takashi Umeda, Yuji Ashikawa, Chiho Suzuki-Minakuchi, Yoshiaki Kawano, Zui Fujimoto, Kazunori Okada, Hisakazu Yamane, Hideaki Nojiri

Abstract read
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In one paragraph

Article in Acta crystallographica. Section F, Structural biology communications, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

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4 · The record

Corrections and comments

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5 · Who and what money

Authors and funding

10 authors.

Jun MatsuzawaBiotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Hiroki AikawaBiotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Takashi UmedaBiotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Yuji AshikawaBiotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Chiho Suzuki-MinakuchiBiotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Yoshiaki KawanoSR Life Science Instrumentation Unit, Research Infrastructure Group, Advanced Photon Technology Division, RIKEN SPring-8 Center, RIKEN Harima Branch, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5148, Japan.
Zui FujimotoBiomolecular Research Unit, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan.
Kazunori OkadaBiotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Hisakazu YamaneDepartment of Biosciences, Teikyo University, 1-1 Toyosatodai, Utsunomiya, Tochigi 320-0003, Japan.
Hideaki NojiriBiotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The initial reaction in bacterial carbazole degradation is catalyzed by carbazole 1,9a-dioxygenase, which consists of terminal oxygenase (Oxy), ferredoxin (Fd) and ferredoxin reductase components. The electron-transfer complex between reduced Oxy and oxidized Fd was crystallized at 293 K using the hanging-drop vapour-diffusion method with PEG 3350 as the precipitant under anaerobic conditions. The crystal diffracted to a maximum resolution of 2.25 Å and belonged to space group P21, with unit-cell parameters a = 97.3, b = 81.6, c = 116.2 Å, α = γ = 90, β = 100.1°. The VM value is 2.85 Å(3) Da(-1), indicating a solvent content of 56.8%.

Indexed as

Bacterial ProteinsCatalytic DomainCrystallizationCrystallography, X-RayDioxygenasesFerredoxinsOxidation-ReductionPseudomonasBacterial Proteinscarbazole 1,9a-dioxygenaseDioxygenasesFerredoxinscarbazole 1,9a-dioxygenaseelectron-transfer complexferredoxinRieske nonhaem iron oxygenaseterminal oxygenase

Identifiers

PMID25286950
PMCPMC4188090

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.