ArticleJournal of structural biology2014
Efficient cryoprotection of macromolecular crystals using vapor diffusion of volatile alcohols.
Article in Journal of structural biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed, 12 citations in OpenAlex.
- Automated gradient equilibration of macromolecular crystals to new solution conditions.Acta crystallographica. Section F, Structural biology communications · 2025Article
- A drug-discovery-oriented non-invasive protocol for protein crystal cryoprotection by dehydration, with application for crystallization screening.Journal of applied crystallography · 2022Article
- Alpha-Lipoic Acid Can Overcome The Reduced Developmental Competency Induced by Alcohol Toxicity during Ovine Oocyte Maturation.Cell journal · 2021Article
- Structural Insights into the Recognition of Mono- and Diacetylated Histones by the ATAD2B Bromodomain.Journal of medicinal chemistry · 2020Article
- A comparison of gas stream cooling and plunge cooling of macromolecular crystals.Journal of applied crystallography · 2019Article
- The impact of cryosolution thermal contraction on proteins and protein crystals: volumes, conformation and order.Acta crystallographica. Section D, Structural biology · 2018Article
- Effects of protein-crystal hydration and temperature on side-chain conformational heterogeneity in monoclinic lysozyme crystals.Acta crystallographica. Section D, Structural biology · 2018Article
- Multicomponent mixtures for cryoprotection and ligand solubilization.Biotechnology reports (Amsterdam, Netherlands) · 2015Article
- Practical macromolecular cryocrystallography.Acta crystallographica. Section F, Structural biology communications · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Macromolecular X-ray crystallography, usually done at cryogenic temperature to limit radiation damage, often requires liquid cryoprotective soaking that can be labor intensive and damaging to crystals. Here we describe a method for cryoprotection that uses vapor diffusion of volatile cryoprotective agents into loop-mounted crystals. The crystal is mounted into a vial containing a small volume of an alcohol-based cryosolution. After a short incubation with the looped crystal sitting in the cryosolution vapor, the crystal is transferred directly from the vial into the cooling medium. Effective for several different protein crystals, the approach obviates the need for liquid soaking and opens up a heretofore underutilized class of cryoprotective agents for macromolecular crystallography.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.