ArticlePloS one2014
Expression, purification, and structural insights for the human uric acid transporter, GLUT9, using the Xenopus laevis oocytes system.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 39 citations in OpenAlex.
- ABCG2 transporter: Structural and functional associations with gout (Review).International journal of molecular medicine · 2026Review
- NewGenes · 2023Article
- Pathogenic Variants ofInternational journal of molecular sciences · 2023Article
- The in-silico evaluation of important GLUT9 residue for uric acid transport based on renal hypouricemia type 2.Chemico-biological interactions · 2023Article
- Article
- Comparative Transcriptomics and Proteomics of Cancer Cell Lines Cultivated by Physiological and Commercial Media.Biomolecules · 2022Article
- Glycome Profiling of Cancer Cell Lines Cultivated in Physiological and Commercial Media.Biomolecules · 2022Article
- Characterization of a Compound Heterozygous SLC2A9 Mutation That Causes Hypouricemia.Biomedicines · 2021Article
- Chicken serum uric acid level is regulated by glucose transporter 9.Animal bioscience · 2021Article
- Contribution of SLC22A12 on hypouricemia and its clinical significance for screening purposes.Scientific reports · 2019Article
- The effect of polymorphism of uric acid transporters on uric acid transport.Journal of nephrology · 2019Review
- Insights into Substrate and Inhibitor Selectivity among Human GLUT Transporters through Comparative Modeling and Molecular Docking.ACS omega · 2019Article
- Large-scale whole-exome sequencing association studies identify rare functional variants influencing serum urate levels.Nature communications · 2018Article
- Identification of essential amino acids for glucose transporter 5 (GLUT5)-mediated fructose transport.The Journal of biological chemistry · 2018Article
- Human Mutations in SLC2A9 (Glut9) Affect Transport Capacity for Urate.Frontiers in physiology · 2018Article
- Effects of Chicory on Serum Uric Acid, Renal Function, and GLUT9 Expression in Hyperuricaemic Rats with Renal Injury andEvidence-based complementary and alternative medicine : eCAM · 2018Article
- Uric acid in the pathogenesis of metabolic, renal, and cardiovascular diseases: A review.Journal of advanced research · 2017Review
- Identification of Key Residues for Urate Specific Transport in Human Glucose Transporter 9 (hSLC2A9).Scientific reports · 2017Article
- Fasting plasma glucose and serum uric acid levels in a general Chinese population with normal glucose tolerance: A U-shaped curve.PloS one · 2017Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The urate transporter, GLUT9, is responsible for the basolateral transport of urate in the proximal tubule of human kidneys and in the placenta, playing a central role in uric acid homeostasis. GLUT9 shares the least homology with other members of the glucose transporter family, especially with the glucose transporting members GLUT1-4 and is the only member of the GLUT family to transport urate. The recently published high-resolution structure of XylE, a bacterial D-xylose transporting homologue, yields new insights into the structural foundation of this GLUT family of proteins. While this represents a huge milestone, it is unclear if human GLUT9 can benefit from this advancement through subsequent structural based targeting and mutagenesis. Little progress has been made toward understanding the mechanism of GLUT9 since its discovery in 2000. Before work can begin on resolving the mechanisms of urate transport we must determine methods to express, purify and analyze hGLUT9 using a model system adept in expressing human membrane proteins. Here, we describe the surface expression, purification and isolation of monomeric protein, and functional analysis of recombinant hGLUT9 using the Xenopus laevis oocyte system. In addition, we generated a new homology-based high-resolution model of hGLUT9 from the XylE crystal structure and utilized our purified protein to generate a low-resolution single particle reconstruction. Interestingly, we demonstrate that the functional protein extracted from the Xenopus system fits well with the homology-based model allowing us to generate the predicted urate-binding pocket and pave a path for subsequent mutagenesis and structure-function studies.
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