ArticleJournal of visualized experiments : JoVE2014
Real-time imaging of axonal transport of quantum dot-labeled BDNF in primary neurons.
Article in Journal of visualized experiments : JoVE, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 36 citations in OpenAlex.
- Human Dorsal Root Ganglia Neuronal Cell Line to Study Nociceptive Signaling: A New Pipeline for Pain Therapy.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026Article
- Review
- Article
- Failure to Thrive: Impaired BDNF Transport along the Cortical-Striatal Axis in Mouse Q140 Neurons of Huntington's Disease.Biology · 2023Article
- Molecular-Scale Dynamics of Long Range Retrograde Brain-Derived Neurotrophic Factor Transport Shaped by Cellular Spatial Context.Frontiers in neuroscience · 2022Article
- Multimodal single-molecule microscopy with continuously controlled spectral resolution.Biophysical reports · 2021Article
- Laser-Induced Shockwave (LIS) to Study Neuronal CaFrontiers in bioengineering and biotechnology · 2021Article
- Histone deacetylase 6 inhibition rescues axonal transport impairments and prevents the neurotoxicity of HIV-1 envelope protein gp120.Cell death & disease · 2019Article
- Accumbens brain-derived neurotrophic factor (BDNF) transmission inhibits cocaine seeking.Addiction biology · 2019Article
- Compartmentalized Devices as Tools for Investigation of Human Brain Network Dynamics.Developmental dynamics : an official publication of the American Association of Anatomists · 2019Review
- The Need for Physiological Micro-Nanofluidic Systems of the Brain.Frontiers in bioengineering and biotechnology · 2019Review
- T-complex protein 1-ring complex enhances retrograde axonal transport by modulating tau phosphorylation.Traffic (Copenhagen, Denmark) · 2018Article
- Inhibition of RhoA reduces propofol-mediated growth cone collapse, axonal transport impairment, loss of synaptic connectivity, and behavioural deficits.British journal of anaesthesia · 2018Article
- Aberrant GlyRS-HDAC6 interaction linked to axonal transport deficits in Charcot-Marie-Tooth neuropathy.Nature communications · 2018Article
- Synuclein impairs trafficking and signaling of BDNF in a mouse model of Parkinson's disease.Scientific reports · 2017Article
- Enhanced neuroinvasion by smaller, soluble prions.Acta neuropathologica communications · 2017Article
- TRiC subunits enhance BDNF axonal transport and rescue striatal atrophy in Huntington's disease.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Amyloid precursor protein-mediated endocytic pathway disruption induces axonal dysfunction and neurodegeneration.The Journal of clinical investigation · 2016Article
- Functional Impact of Corticotropin-Releasing Factor Exposure on Tau Phosphorylation and Axon Transport.PloS one · 2016Article
- Microfluidic Organ/Body-on-a-Chip Devices at the Convergence of Biology and Microengineering.Sensors (Basel, Switzerland) · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
BDNF plays an important role in several facets of neuronal survival, differentiation, and function. Structural and functional deficits in axons are increasingly viewed as an early feature of neurodegenerative diseases, including Alzheimer's disease (AD) and Huntington's disease (HD). As yet unclear is the mechanism(s) by which axonal injury is induced. We reported the development of a novel technique to produce biologically active, monobiotinylated BDNF (mBtBDNF) that can be used to trace axonal transport of BDNF. Quantum dot-labeled BDNF (QD-BDNF) was produced by conjugating quantum dot 655 to mBtBDNF. A microfluidic device was used to isolate axons from neuron cell bodies. Addition of QD-BDNF to the axonal compartment allowed live imaging of BDNF transport in axons. We demonstrated that QD-BDNF moved essentially exclusively retrogradely, with very few pauses, at a moving velocity of around 1.06 μm/sec. This system can be used to investigate mechanisms of disrupted axonal function in AD or HD, as well as other degenerative disorders.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.