ArticleJournal of visualized experiments : JoVE2014
Isolation and culture of dissociated sensory neurons from chick embryos.
Article in Journal of visualized experiments : JoVE, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 14 citations in OpenAlex.
- Contribution of gelsolin and its secretory isoform to the migratory properties of neural crest and DRG cells in the chicken embryo.Frontiers in molecular neuroscience · 2026Article
- Filamented hydrogels as tunable conduits for guiding neurite outgrowth.Materials today. Bio · 2025Article
- Non-integrin laminin receptor (LamR) plays a role in axonal outgrowth from chicken DRG via modulating the Akt and Erk signaling.Frontiers in cell and developmental biology · 2024Article
- Editorial: Peripheral nerve anatomy in health and disease.Journal of anatomy · 2022Article
- Optimized Decellularization Protocol for Large Peripheral Nerve Segments: Towards Personalized Nerve Bioengineering.Bioengineering (Basel, Switzerland) · 2022Article
- Characteristics of neural growth and cryopreservation of the dorsal root ganglion using three-dimensional collagen hydrogel cultureNeural regeneration research · 2021Article
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- Intracellular G-actin targeting of peripheral sensory neurons by the multifunctional engineered protein C2C confers relief from inflammatory pain.Scientific reports · 2020Article
- Synergistic effect of CNTF and GDNF on directed neurite growth in chick embryo dorsal root ganglia.PloS one · 2020Article
- Expression of pseudorabies virus-encoded long noncoding RNAs in epithelial cells and neurons.Journal of neurovirology · 2018Article
- General Method for Generating Circular Gradients of Active Proteins on Nanofiber Scaffolds Sought for Wound Closure and Related Applications.ACS applied materials & interfaces · 2018Article
- Differentiation of Bone Marrow Stem Cells into Schwann Cells for the Promotion of Neurite Outgrowth on Electrospun Fibers.ACS applied materials & interfaces · 2017Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Neurons are multifaceted cells that carry information essential for a variety of functions including sensation, motor movement, learning, and memory. Studying neurons in vivo can be challenging due to their complexity, their varied and dynamic environments, and technical limitations. For these reasons, studying neurons in vitro can prove beneficial to unravel the complex mysteries of neurons. The well-defined nature of cell culture models provides detailed control over environmental conditions and variables. Here we describe how to isolate, dissociate, and culture primary neurons from chick embryos. This technique is rapid, inexpensive, and generates robustly growing sensory neurons. The procedure consistently produces cultures that are highly enriched for neurons and has very few non-neuronal cells (less than 5%). Primary neurons do not adhere well to untreated glass or tissue culture plastic, therefore detailed procedures to create two distinct, well-defined laminin-containing substrata for neuronal plating are described. Cultured neurons are highly amenable to multiple cellular and molecular techniques, including co-immunoprecipitation, live cell imagining, RNAi, and immunocytochemistry. Procedures for double immunocytochemistry on these cultured neurons have been optimized and described here.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.