ArticlePloS one2014
Honey bee hemocyte profiling by flow cytometry.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 47 citations in OpenAlex.
- Immune response in honey bees (Apis mellifera) following humic substance feeding.Veterinary research communications · 2026Article
- Clodronate Liposomes Untangle the Role of Hemocytes in <italic>Apis mellifera</italic> Response to Temperature Variation and Microbial Infection.Journal of innate immunity · 2026Article
- Clodronate liposomes untangle the role of hemocytes inbioRxiv : the preprint server for biology · 2025Article
- Modeling seasonal immune dynamics of honey bee (Apis mellifera L.) response to injection of heat-killed Serratia marcescens.PloS one · 2024Article
- Immunological responses and gut microbial shifts inFrontiers in microbiology · 2023Article
- Intracellular cytokine detection based on flow cytometry in hemocytes from Galleria mellonella larvae: A new protocol.PloS one · 2022Article
- Article
- Morphological and Ultrastructural Characterization of Hemocytes in an Insect Model, the HematophagousInsects · 2021Article
- Improved mitochondrial function corrects immunodeficiency and impaired respiration in neonicotinoid exposed bumblebees.PloS one · 2021Article
- Virus and dsRNA-triggered transcriptional responses reveal key components of honey bee antiviral defense.Scientific reports · 2017Article
- Immunosuppression in Honeybee Queens by the Neonicotinoids Thiacloprid and Clothianidin.Scientific reports · 2017Article
- Hemocyte-mediated phagocytosis differs between honey bee (Apis mellifera) worker castes.PloS one · 2017Article
- Sepsis and Hemocyte Loss in Honey Bees (Apis mellifera) Infected with Serratia marcescens Strain Sicaria.PloS one · 2016Article
- Antiviral Defense Mechanisms in Honey Bees.Current opinion in insect science · 2015Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Multiple stress factors in honey bees are causing loss of bee colonies worldwide. Several infectious agents of bees are believed to contribute to this problem. The mechanisms of honey bee immunity are not completely understood, in part due to limited information about the types and abundances of hemocytes that help bees resist disease. Our study utilized flow cytometry and microscopy to examine populations of hemolymph particulates in honey bees. We found bee hemolymph includes permeabilized cells, plasmatocytes, and acellular objects that resemble microparticles, listed in order of increasing abundance. The permeabilized cells and plasmatocytes showed unexpected differences with respect to properties of the plasma membrane and labeling with annexin V. Both permeabilized cells and plasmatocytes failed to show measurable mitochondrial membrane potential by flow cytometry using the JC-1 probe. Our results suggest hemolymph particulate populations are dynamic, revealing significant differences when comparing individual hive members, and when comparing colonies exposed to diverse conditions. Shifts in hemocyte populations in bees likely represent changing conditions or metabolic differences of colony members. A better understanding of hemocyte profiles may provide insight into physiological responses of honey bees to stress factors, some of which may be related to colony failure.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.