Evidence map›Paper›PMID 25285608›Full record

ArticleJournal of visualized experiments : JoVE2014

Isolating primary melanocyte-like cells from the mouse heart.

Hayoung Hwang, Fang Liu, Mark D Levin, Vickas V Patel

Open access · bronzeAbstract readVideo-Audio Media
In one paragraph

Article in Journal of visualized experiments : JoVE, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.5field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Hayoung HwangPenn Cardiovascular Institute, Perelman School of Medicine, University of Pennsylvania.
Fang LiuPenn Cardiovascular Institute, Perelman School of Medicine, University of Pennsylvania.
Mark D LevinPenn Cardiovascular Institute, Perelman School of Medicine, University of Pennsylvania.
Vickas V PatelPenn Cardiovascular Institute, Perelman School of Medicine, University of Pennsylvania ; patelv@mail.med.upenn.edu.
University of Pennsylvania · US

Funding

The Contribution of Melanocyte-like Cells to Atrial Function and DevelopmentR01HL105734 · NHLBI · UNIVERSITY OF PENNSYLVANIA · PI CHEN, XIONGWEN · 2011 to 2015
$2.3M
A MODEL OF ATRIAL FIBRILATION TRIGGERSK08HL094748 · NHLBI · WASHINGTON UNIVERSITY · PI LEVIN, MARK DAVID · 2011 to 2014
$491k
NHLBI NIH HHS K08 HL094748NHLBI NIH HHS R01 HL105734
6 · The paper itself

Abstract

We identified a novel population of melanocyte-like cells (also known as cardiac melanocytes) in the hearts of mice and humans that contribute to atrial arrhythmia triggers in mice. To investigate the electrical and biological properties of cardiac melanocytes we developed a procedure to isolate them from mouse hearts that we derived from those designed to isolate neonatal murine cardiomyocytes. In order to obtain healthier cardiac melanocytes suitable for more extensive patch clamp or biochemical studies, we developed a refined procedure for isolating and plating cardiac melanocytes based on those originally designed to isolate cutaneous melanocytes. The refined procedure is demonstrated in this review and produces larger numbers of healthy melanocyte-like cells that can be plated as a pure population or with cardiomyocytes.

Indexed as

AnimalsAtrial FibrillationCytological TechniquesFemaleHumansMaleMelanocytesMiceMyocardium

Identifiers

PMID25285608
PMCPMC4828152
OpenAlexW2007744097

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.