ArticleJournal of visualized experiments : JoVE2014
In vivo and in vitro rearing of entomopathogenic nematodes (Steinernematidae and Heterorhabditidae).
Article in Journal of visualized experiments : JoVE, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 44 citations in OpenAlex.
- Glucosinolate hydrolysis products suppress entomopathogenic nematodes in vitro but do not protect sequestering flea beetle larvae in vivo.Pest management science · 2026Article
- Evolution of parasitism-related traits in nematodes.bioRxiv : the preprint server for biology · 2025Article
- Storage Capacity of Entomopathogenic Nematodes in BarricadeJournal of nematology · 2025Article
- Comparative Virulence of Entomopathogenic Nematodes to the Small Hive Beetle (Journal of nematology · 2025Article
- Entomopathogenic Nematode Species Vary in Their Behavior and Virulence in Response to Cardiac Glycosides Within and Around Insect Hosts.Journal of chemical ecology · 2025Article
- Green and red fluorescent strains ofmicroPublication biology · 2024Article
- Involvement of a novel Pseudomonas protegens strain associated with entomopathogenic nematode infective juveniles in insect pathogenesis.Pest management science · 2022Article
- Apex Predator Nematodes and Meso-Predator Bacteria Consume Their Basal Insect Prey through Discrete Stages of Chemical Transformations.mSystems · 2022Article
- A Review of Entomopathogenic Nematodes as a Biological Control Agent for Red Palm Weevil,Insects · 2022Review
- Transcriptomic Analysis ofFrontiers in physiology · 2022Article
- Comparison of Biological Activity of Field Isolates ofInsects · 2021Article
- Natural enemies of herbivores maintain their biological control potential under short-term exposure to future COEcology and evolution · 2021Article
- Bioturbation by endogeic earthworms facilitates entomopathogenic nematode movement toward herbivore-damaged maize roots.Scientific reports · 2020Article
- The differential strain virulence of the candidate toxins of3 Biotech · 2020Article
- Plant defense resistance in natural enemies of a specialist insect herbivore.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
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- Fitness costs of symbiont switching using entomopathogenic nematodes as a model.BMC evolutionary biology · 2017Article
- Infective Juveniles of the Entomopathogenic Nematode Steinernema scapterisci Are Preferentially Activated by Cricket Tissue.PloS one · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Entomopathogenic nematodes (EPN) (Steinernematidae and Heterorhabditidae) have a mutualistic partnership with Gram-negative Gamma-Proteobacteria in the family Enterobacteriaceae. Xenorhabdus bacteria are associated with steinernematids nematodes while Photorhabdus are symbionts of heterorhabditids. Together nematodes and bacteria form a potent insecticidal complex that kills a wide range of insect species in an intimate and specific partnership. Herein, we demonstrate in vivo and in vitro techniques commonly used in the rearing of these nematodes under laboratory conditions. Furthermore, these techniques represent key steps for the successful establishment of EPN cultures and also form the basis for other bioassays that utilize these organisms for research. The production of aposymbiotic (symbiont-free) nematodes is often critical for an in-depth and multifaceted approach to the study of symbiosis. This protocol does not require the addition of antibiotics and can be accomplished in a short amount of time with standard laboratory equipment. Nematodes produced in this manner are relatively robust, although their survivorship in storage may vary depending on the species used. The techniques detailed in this presentation correspond to those described by various authors and refined by P. Stock's Laboratory, University of Arizona (Tucson, AZ, USA). These techniques are distinct from the body of techniques that are used in the mass production of these organisms for pest management purposes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.