ArticleJournal of visualized experiments : JoVE2014
Generation of transgenic Hydra by embryo microinjection.
Article in Journal of visualized experiments : JoVE, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 33 citations in OpenAlex.
- A molecular, spatial and regulatory atlas of the Hydra vulgaris nervous system.Development (Cambridge, England) · 2025Article
- The Bioelectrics of Immortality and Mortality in Cold-SensitiveBioelectricity · 2025Article
- The dynamic genomes of Hydra and the anciently active repeat complement of animal chromosomes.Genome biology · 2025Article
- Terminal differentiation precedes functional circuit integration in the peduncle neurons in regenerating Hydra vulgaris.Neural development · 2024Article
- Automatic monitoring of neural activity with single-cell resolution in behaving Hydra.Scientific reports · 2024Article
- Article
- Automatic monitoring of whole-body neural activity in behavingbioRxiv : the preprint server for biology · 2023Article
- Hydra vulgaris shows stable responses to thermal stimulation despite large changes in the number of neurons.iScience · 2021Article
- Cell Sorting in Hydra vulgaris Arises from Differing Capacities for Epithelialization between Cell Types.Current biology : CB · 2020Article
- Mouth Function Determines the Shape Oscillation Pattern in Regenerating Hydra Tissue Spheres.Biophysical journal · 2019Article
- Insights into regeneration tool box: An animal model approach.Developmental biology · 2019Review
- Stem cell differentiation trajectories inScience (New York, N.Y.) · 2019Article
- Transgenesis in Hydra to characterize gene function and visualize cell behavior.Nature protocols · 2019Article
- Linalool acts as a fast and reversible anesthetic in Hydra.PloS one · 2019Article
- Neuronal photoactivation through second-harmonic near-infrared absorption by gold nanoparticles.Light, science & applications · 2018Article
- Non-overlapping Neural Networks in Hydra vulgaris.Current biology : CB · 2017Article
- Thoughts on a very acidic symbiosome.Frontiers in microbiology · 2015Article
- An efficient strategy for generation of transgenic mice by lentiviral transduction of male germline stem cells in vivo.Journal of animal science and biotechnology · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 3 institutions in 1 country.
Funding
Abstract
As a member of the phylum Cnidaria, the sister group to all bilaterians, Hydra can shed light on fundamental biological processes shared among multicellular animals. Hydra is used as a model for the study of regeneration, pattern formation, and stem cells. However, research efforts have been hampered by lack of a reliable method for gene perturbations to study molecular function. The development of transgenic methods has revitalized the study of Hydra biology(1). Transgenic Hydra allow for the tracking of live cells, sorting to yield pure cell populations for biochemical analysis, manipulation of gene function by knockdown and over-expression, and analysis of promoter function. Plasmid DNA injected into early stage embryos randomly integrates into the genome early in development. This results in hatchlings that express transgenes in patches of tissue in one or more of the three lineages (ectodermal epithelial, endodermal epithelial, or interstitial). The success rate of obtaining a hatchling with transgenic tissue is between 10% and 20%. Asexual propagation of the transgenic hatchling is used to establish a uniformly transgenic line in a particular lineage. Generating transgenic Hydra is surprisingly simple and robust, and here we describe a protocol that can be easily implemented at low cost.
Indexed as
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.