Evidence map›Paper›PMID 25285127›Full record

ArticleThe Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale2014

Evaluation of amplification targets for the specific detection of Bordetella pertussis using real-time polymerase chain reaction.

Mohammad Rubayet Hasan, Rusung Tan, Ghada N Al-Rawahi, Eva Thomas, Peter Tilley

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.1field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 12 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 2 countries.

Mohammad Rubayet HasanDivision of Microbiology, Virology and Infection Control, Department of Pathology, Children's and Women's Health Centre of BC; ; Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, British Columbia;
Rusung TanDivision of Microbiology, Virology and Infection Control, Department of Pathology, Children's and Women's Health Centre of BC; ; Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, British Columbia; ; Sidra Medical and Research Center, Doha, Qatar.
Ghada N Al-RawahiDivision of Microbiology, Virology and Infection Control, Department of Pathology, Children's and Women's Health Centre of BC; ; Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, British Columbia;
Eva ThomasDivision of Microbiology, Virology and Infection Control, Department of Pathology, Children's and Women's Health Centre of BC; ; Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, British Columbia;
Peter TilleyDivision of Microbiology, Virology and Infection Control, Department of Pathology, Children's and Women's Health Centre of BC; ; Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, British Columbia;
Children's & Women's Health Centre of British Columbia · CAUniversity of British Columbia · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundBordetella pertussis infections continue to be a major public health challenge in Canada. Polymerase chain reaction (PCR) assays to detect B pertussis are typically based on the multicopy insertion sequence IS481, which offers high sensitivity but lacks species specificity.

methodsA novel B pertussis real-time PCR assay based on the porin gene was tested in parallel with several previously published assays that target genes such as IS481, ptx-promoter, pertactin and a putative thialase. The assays were evaluated using a reference panel of common respiratory bacteria including different Bordetella species and 107 clinical nasopharyngeal specimens. Discrepant results were confirmed by sequencing the PCR products.

resultsAnalytical sensitivity was highest for the assay targeting the IS481 element; however, the assay lacked specificity for B pertussis in the reference panel and in the clinical samples. False-positive results were also observed with assays targeting the ptx-promoter and pertactin genes. A PCR assay based on the thialase gene was highly specific but failed to detect all reference strains of B pertussis. However, a novel assay targeting the porin gene demonstrated high specificity for B pertussis both in the reference panel and in clinical samples and, based on sequence-confirmed results, correctly predicted all B pertussis-positive cases in clinical samples. According to Probit regression analysis, the 95% detection limit of the new assay was 4 colony forming units/reaction.

conclusionA novel porin assay for B pertussis demonstrated superior performance and may be useful for improved molecular detection of B pertussis in clinical specimens.

Indexed as

Bordetella pertussisIS481 elementPertactin genePorin genePtx-promoterReal-time PCR

Identifiers

PMID25285127
PMCPMC4173943
OpenAlexW2224029405

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.