ArticleIndian journal of pharmaceutical sciences2014
Standardization of in vitro Cell-based Model for Renal Ischemia and Reperfusion Injury.
Article in Indian journal of pharmaceutical sciences, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 27 citations in OpenAlex.
- The Current Application Prospects of Nanomedicine in Renal Ischemia-Reperfusion Injury.International journal of nanomedicine · 2026Review
- Article
- Sphingosine 1-phosphate protective effect on human proximal tubule cells submitted to an in vitro ischemia model: the role of JAK2/STAT3.Journal of physiology and biochemistry · 2024Article
- Article
- Modeling Central Nervous System Injury In Vitro: Current Status and Promising Future Strategies.Biomedicines · 2022Review
- Ischemic Stroke, Lessons from the Past towards Effective Preclinical Models.Biomedicines · 2022Review
- Therapeutical Significance of Serpina3n Subsequent Cerebral Ischemia via Cytotoxic Granzyme B Inactivation.BioMed research international · 2022Article
- Modeling ischemic stroke in a triculture neurovascular unit on-a-chip.Fluids and barriers of the CNS · 2021Article
- The Protective Effect of Anthocyanins Extracted fromMediators of inflammation · 2021Article
- Plk2 Regulated by miR-128 Induces Ischemia-Reperfusion Injury in Cardiac Cells.Molecular therapy. Nucleic acids · 2020Article
- Trib1 Contributes to Recovery From Ischemia/Reperfusion-Induced Acute Kidney Injury by Regulating the Polarization of Renal Macrophages.Frontiers in immunology · 2020Article
- Aristolochic acid I determine the phenotype and activation of macrophages in acute and chronic kidney disease.Scientific reports · 2018Article
- Social interaction modulates the neuroinflammatory response to global cerebral ischemia in male mice.Brain research · 2017Article
- Administration of hydrogen sulfide protects ischemia reperfusion-induced acute kidney injury by reducing the oxidative stress.Irish journal of medical science · 2016Article
- Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Renal ischemia reperfusion injury contributes patho-physiological imbalance of acute renal failure that comprises of generation of reactive oxygen species, nitric oxide and peroxynitrite and inflammation involving cytokine/adhesion molecule cascade, finally leads to cell death. Oxygen deprival associated with ischemia that in turn lead to decline ATP production is the characteristic feature usually addressed in the development of in vitro cell based ischemic model. In order to create oxygen deficit in the cell lines different approaches like chemical induction, enzymatic induction and anaerobic chamber models are widely used. However efficiencies of these models were varied and the present study was aimed to compare the suitability of these models in creating in vitro ischemia reperfusion in cell culture. In the chemical induced method we used different concentrations of rotenone, antimycin and sodium azide to inhibit electron transport chain and thereby reduced the ATP production, measured indirectly by cell viability assay. Among the chemical induced model, antimycin mediated cell injury was more reliable for ischemia reperfusion study. In the enzymatic model, comprises of glucose oxidase (3mM/s) and catalase (998 s(-1) at 10:1 ratio) was used and found to be best among the three approaches as it can create injury in short experimental time and are reproducible. However anaerobic chamber method was not suitable for ischemia reperfusion study as it need more time to induce significant cell injury.
Indexed as
Identifiers
25284933PMC4171872W1554634440What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.