ArticleThe Plant journal : for cell and molecular biology2015
Probing formation of cargo/importin-α transport complexes in plant cells using a pathogen effector.
Article in The Plant journal : for cell and molecular biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 53 citations in OpenAlex.
- Structures of Nicotiana benthamiana importin-α1 complexes reveal mechanisms for plant virus NLS recognition.The Plant journal : for cell and molecular biology · 2026Article
- The importin-alpha superfamily engages in ethylene signaling by shuttling ETHYLENE INSENSITIVE 2 from the endoplasmic reticulum to the nucleus.The FEBS journal · 2026Article
- Appropriate reduction of importin-α gene expression enhances yellow dwarf disease resistance in common wheat.Plant biotechnology journal · 2024Article
- Tuning the Wavelength: Manipulation of Light Signaling to Control Plant Defense.International journal of molecular sciences · 2023Review
- Identification of the Karyopherin Superfamily in Maize and Its Functional Cues in Plant Development.International journal of molecular sciences · 2022Review
- Exploiting breakdown in nonhost effector-target interactions to boost host disease resistance.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- OsWRKY62 and OsWRKY76 Interact with Importin α1s for Negative Regulation of Defensive Responses in Rice Nucleus.Rice (New York, N.Y.) · 2022Article
- A Novel Core Effector Vp1 Promotes Fungal Colonization and Virulence ofJournal of fungi (Basel, Switzerland) · 2021Article
- Importin-αs are required for the nuclear localization and function of the Plasmopara viticola effector PvAVH53.Horticulture research · 2021Article
- Nucleocytoplasmic Communication in Healthy and Diseased Plant Tissues.Frontiers in plant science · 2021Review
- A Poplar Rust Effector Protein Associates with Protein Disulfide Isomerase and Enhances Plant Susceptibility.Biology · 2020Article
- Comparative study of the interactions between fungal transcription factor nuclear localization sequences with mammalian and fungal importin-alpha.Scientific reports · 2020Article
- TPX2-LIKE PROTEIN3 Is the Primary Activator of α-Aurora Kinases and Is Essential for Embryogenesis.Plant physiology · 2019Article
- Identification of Domains and Factors Involved in MINIYO Nuclear Import.Frontiers in plant science · 2019Article
- Arabidopsis downy mildew effector HaRxL106 suppresses plant immunity by binding to RADICAL-INDUCED CELL DEATH1.The New phytologist · 2018Article
- MOS6 and TN13 in plant immunity.Plant signaling & behavior · 2018Article
- Nuclear Import ofFrontiers in plant science · 2018Article
- Nucleoporin-Regulated MAP Kinase Signaling in Immunity to a Necrotrophic Fungal Pathogen.Plant physiology · 2016Article
- Identification of potential cargo proteins of transportin protein AtTRN1 in Arabidopsis thaliana.Plant cell reports · 2016Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
Abstract
Importin-αs are essential adapter proteins that recruit cytoplasmic proteins destined for active nuclear import to the nuclear transport machinery. Cargo proteins interact with the importin-α armadillo repeat domain via nuclear localization sequences (NLSs), short amino acids motifs enriched in Lys and Arg residues. Plant genomes typically encode several importin-α paralogs that can have both specific and partially redundant functions. Although some cargos are preferentially imported by a distinct importin-α it remains unknown how this specificity is generated and to what extent cargos compete for binding to nuclear transport receptors. Here we report that the effector protein HaRxL106 from the oomycete pathogen Hyaloperonospora arabidopsidis co-opts the host cell's nuclear import machinery. We use HaRxL106 as a probe to determine redundant and specific functions of importin-α paralogs from Arabidopsis thaliana. A crystal structure of the importin-α3/MOS6 armadillo repeat domain suggests that five of the six Arabidopsis importin-αs expressed in rosette leaves have an almost identical NLS-binding site. Comparison of the importin-α binding affinities of HaRxL106 and other cargos in vitro and in plant cells suggests that relatively small affinity differences in vitro affect the rate of transport complex formation in vivo. Our results suggest that cargo affinity for importin-α, sequence variation at the importin-α NLS-binding sites and tissue-specific expression levels of importin-αs determine formation of cargo/importin-α transport complexes in plant cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.