ArticleThe Journal of comparative neurology2015
Moniliform deformation of retinal ganglion cells by formaldehyde-based fixatives.
Article in The Journal of comparative neurology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed, 14 citations in OpenAlex.
- Retinal Ganglion Cell Subtypes and Their Vulnerability in Glaucoma.Methods in molecular biology (Clifton, N.J.) · 2025Article
- High fidelity fiber orientation density functions from fiber ball imaging.NMR in biomedicine · 2022Article
- Volumetric super-resolution imaging by serial ultrasectioning and stochastic optical reconstruction microscopy in mouse neural tissue.STAR protocols · 2021Article
- Article
- Dopaminergic amacrine cell number, plexus density, and dopamine content in the mouse retina: Strain differences and effects of Bax gene disruption.Experimental eye research · 2018Article
- Cellular and Molecular Analysis of Dendritic Morphogenesis in a Retinal Cell Type That Senses Color Contrast and Ventral Motion.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- Somatic and neuritic spines on tyrosine hydroxylase-immunopositive cells of rat retina.The Journal of comparative neurology · 2017Article
- Time-Lapse Retinal Ganglion Cell Dendritic Field Degeneration Imaged in Organotypic Retinal Explant Culture.Investigative ophthalmology & visual science · 2016Article
- Fixation strategies for retinal immunohistochemistry.Progress in retinal and eye research · 2015Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Protocols for characterizing cellular phenotypes commonly use chemical fixatives to preserve anatomical features, mechanically stabilize tissue, and stop physiological responses. Formaldehyde, diluted in either phosphate-buffered saline or phosphate buffer, has been widely used in studies of neurons, especially in conjunction with dyes and antibodies. However, previous studies have found that these fixatives induce the formation of bead-like varicosities in the dendrites and axons of brain and spinal cord neurons. We report here that these formaldehyde formulations can induce bead formation in the dendrites and axons of adult rat and rabbit retinal ganglion cells, and that retinal ganglion cells differ from hippocampal, cortical, cerebellar, and spinal cord neurons in that bead formation is not blocked by glutamate receptor antagonists, a voltage-gated Na(+) channel toxin, extracellular Ca(2+) ion exclusion, or temperature shifts. Moreover, we describe a modification of formaldehyde-based fixatives that prevents bead formation in retinal ganglion cells visualized by green fluorescent protein expression and by immunohistochemistry.
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Registered trials
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