ArticleApplied and environmental microbiology2014
Positive regulation of botulinum neurotoxin gene expression by CodY in Clostridium botulinum ATCC 3502.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 26 citations in OpenAlex.
- Pathogenicity and virulence ofVirulence · 2023Review
- Specific Isolation ofInternational journal of molecular sciences · 2022Article
- Review
- Posttranslational Regulation of Botulinum Neurotoxin Production in Clostridium botulinum Hall A-mSphere · 2021Article
- CRISPR-Cas9-Based Toolkit forFrontiers in microbiology · 2021Article
- Time-course transcriptomics reveals that amino acids catabolism plays a key role in toxinogenesis and morphology in Clostridium tetani.Journal of industrial microbiology & biotechnology · 2020Article
- Tetanus Toxin Synthesis is Under the Control of A Complex Network of Regulatory Genes inToxins · 2020Article
- Review
- Impact of CodY protein on metabolism, sporulation and virulence in Clostridioides difficile ribotype 027.PloS one · 2019Article
- Regulation of Botulinum Neurotoxin Synthesis and Toxin Complex Formation by Arginine and Glucose in Clostridium botulinum ATCC 3502.Applied and environmental microbiology · 2017Article
- Article
- An Integrative Approach to Computational Modelling of the Gene Regulatory Network Controlling Clostridium botulinum Type A1 Toxin Production.PLoS computational biology · 2016Article
- Late-Exponential Gene Expression in codY-Deficient Bacillus anthracis in a Host-Like Environment.Current microbiology · 2016Article
- New Elements To Consider When Modeling the Hazards Associated with Botulinum Neurotoxin in Food.Journal of bacteriology · 2016Review
- The pattern of growth observed for Clostridium botulinum type A1 strain ATCC 19397 is influenced by nutritional status and quorum sensing: a modelling perspective.Pathogens and disease · 2015Article
- NanI Sialidase, CcpA, and CodY Work Together To Regulate Epsilon Toxin Production by Clostridium perfringens Type D Strain CN3718.Journal of bacteriology · 2015Article
- Interactive regulation by the Bacillus subtilis global regulators CodY and ScoC.Molecular microbiology · 2015Article
- CodY regulates expression of the Bacillus subtilis extracellular proteases Vpr and Mpr.Journal of bacteriology · 2015Article
- Role of branched-chain amino acid transport in Bacillus subtilis CodY activity.Journal of bacteriology · 2015Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Botulinum neurotoxin, produced mainly by the spore-forming bacterium Clostridium botulinum, is the most poisonous biological substance known. Here, we show that CodY, a global regulator conserved in low-G+C Gram-positive bacteria, positively regulates the botulinum neurotoxin gene expression. Inactivation of codY resulted in decreased expression of botA, encoding the neurotoxin, as well as in reduced neurotoxin synthesis. Complementation of the codY mutation in trans rescued neurotoxin synthesis, and overexpression of codY in trans caused elevated neurotoxin production. Recombinant CodY was found to bind to a 30-bp region containing the botA transcription start site, suggesting regulation of the neurotoxin gene transcription through direct interaction. GTP enhanced the binding affinity of CodY to the botA promoter, suggesting that CodY-dependent neurotoxin regulation is associated with nutritional status.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.