ArticleMicrobial cell factories2014
Catechol biosynthesis from glucose in Escherichia coli anthranilate-overproducer strains by heterologous expression of anthranilate 1,2-dioxygenase from Pseudomonas aeruginosa PAO1.
Article in Microbial cell factories, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 38 citations in OpenAlex.
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- Metabolic Engineering of Shikimic Acid Biosynthesis Pathway for the Production of Shikimic Acid and Its Branched Products in Microorganisms: Advances and Prospects.Molecules (Basel, Switzerland) · 2022Review
- ComparingProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Plasma Anthranilic Acid and Leptin Levels Predict HAM-D Scores in Depressed Women.International journal of tryptophan research : IJTR · 2021Article
- Catechol-Loading Nanofibrous Membranes for Eco-Friendly Iron Nutrition of Plants.Nanomaterials (Basel, Switzerland) · 2019Article
- Production of Melanins With Recombinant Microorganisms.Frontiers in bioengineering and biotechnology · 2019Review
- Biosynthesis of catechol melanin from glycerol employing metabolically engineered Escherichia coli.Microbial cell factories · 2016Article
- Systems Metabolic Engineering of Escherichia coli.EcoSal Plus · 2016Review
- Metabolic Engineering of Pseudomonas putida KT2440 to Produce Anthranilate from Glucose.Frontiers in microbiology · 2015Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe aromatic compound catechol is used as a precursor of chemical products having multiple applications. This compound is currently manufactured by chemical synthesis from petroleum-derived raw materials. The capacity to produce catechol is naturally present in several microbial species. This knowledge has been applied to the generation of recombinant Escherichia coli strains that can produce catechol from simple carbon sources.
resultsSeveral strains derived from E. coli W3110 trpD9923, a mutant that overproduces anthranilate, were modified by transforming them with an expression plasmid carrying genes encoding anthranilate 1,2-dioxygenase from Pseudomonas aeruginosa PAO1. The additional expression of genes encoding a feedback inhibition resistant version of 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase and transketolase from E. coli, was also evaluated. Generated strains were characterized in complex or minimal medium in shake-flask and fed-batch bioreactor cultures and incubation temperatures ranging from 37 to 28°C. These experiments enabled the identification of culture conditions for the production of 4.47 g/L of catechol with strain W3110 trpD9923, expressing 1,2-dioxygenase, DAHP synthase and transketolase. When considering the amount of glucose consumed, a yield of 16% was calculated, corresponding to 42% of the theoretical maximum as determined by elementary node flux analysis.
conclusionsThis work demonstrates the feasibility of applying metabolic engineering for generating E. coli strains for the production of catechol from glucose via anthranilate. These results are a starting point to further optimize environmentally-compatible production capacity for catechol and derived compounds.
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