Evidence map›Paper›PMID 25281002›Full record

ArticleBiomolecular NMR assignments2015

Backbone assignments of mini-RecA intein with short native exteins and an active N-terminal catalytic cysteine.

C Seth Pearson, Georges Belfort, Marlene Belfort, Alexander Shekhtman

Abstract read
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In one paragraph

Article in Biomolecular NMR assignments, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

C Seth PearsonHoward P. Isermann Department of Chemical and Biological Engineering and The Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, 12180, USA.
Georges BelfortHoward P. Isermann Department of Chemical and Biological Engineering and The Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, 12180, USA.
Marlene BelfortDepartment of Biological Sciences and The RNA Institute, University at Albany, Albany, NY, 12222, USA. mbelfort@albany.edu.
Alexander ShekhtmanDepartment of Chemistry, University at Albany, Albany, NY, 12222, USA.

Funding

SELF-SPLICING INTEINS: FUNCTION, EVOLUTION, APPLICATIONR01GM044844 · NIGMS · WADSWORTH CENTER · PI BELFORT, MARLENE · 1990 to 2019
$8.9M
Modular and Project Based Training to Enhance Rigor and Reproducibility in Research for Diverse Biomedical CareersT32GM067545 · NIGMS · RENSSELAER POLYTECHNIC INSTITUTE · PI VASHISHTH, DEEPAK · 2004 to 2020
$3.0M
Real Time (RT) In-cell NMR technology to study protein interactions in live cellsR01GM085006 · NIGMS · STATE UNIVERSITY OF NEW YORK AT ALBANY · PI SHEKHTMAN, ALEXANDER · 2010 to 2021
$2.4M
NIGMS NIH HHS GM067545NIGMS NIH HHS GM085006NIGMS NIH HHS GM44844NIGMS NIH HHS R01 GM044844NIGMS NIH HHS R01 GM085006NIGMS NIH HHS T32 GM067545
6 · The paper itself

Abstract

The backbone resonance assignments of an engineered splicing-inactive mini-RecA intein based on triple resonance experiments with [(13)C,(15)N]-labeled protein are reported. The construct contains inactivating mutations specifically designed to retain most catalytic residues, especially those that are potentially metal-coordinating. The assignments are essential for protein structure determination of a precursor with an active N-terminal catalytic cysteine and for investigation of the atomic details of splicing.

Indexed as

ExteinsInteinsNuclear Magnetic Resonance, BiomolecularBiocatalysisCysteineProtein EngineeringProton Magnetic Resonance SpectroscopyRec A RecombinasesCysteineRec A RecombinasesInteinProtein splicingTuberculosis

Identifiers

PMID25281002
PMCPMC4385508

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.