Evidence map›Paper›PMID 25279733›Full record

ArticlePloS one2014

Flow characterization of a spinner flask for induced pluripotent stem cell culture application.

Mohd-Zulhilmi Ismadi, Priyanka Gupta, Andreas Fouras, Paul Verma, Sameer Jadhav, Jayesh Bellare, Kerry Hourigan

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed, 1 pooled it
4.6field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed, 1 synthesis or guideline pooled it, 91 citations in OpenAlex.

  1. Pooled it
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  11. In Vitro 3D Modeling of Neurodegenerative Diseases.Bioengineering (Basel, Switzerland) · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

Mohd-Zulhilmi IsmadiDivision of Biological Engineering, Monash University, Melbourne, Victoria, Australia; Department of Mechanical and Aerospace Engineering, Monash University, Melbourne, Victoria, Australia.
Priyanka GuptaDivision of Biological Engineering, Monash University, Melbourne, Victoria, Australia; Department of Chemical Engineering, Monash University, Melbourne, Victoria, Australia; Department of Chemical Engineering, Indian Institute of Technology Bombay, Mumbai, India.
Andreas FourasDivision of Biological Engineering, Monash University, Melbourne, Victoria, Australia; Department of Mechanical and Aerospace Engineering, Monash University, Melbourne, Victoria, Australia.
Paul VermaDivision of Biological Engineering, Monash University, Melbourne, Victoria, Australia; South Australian Research and Development Institute, Rosedale, South Australia, Australia.
Sameer JadhavDepartment of Chemical Engineering, Indian Institute of Technology Bombay, Mumbai, India.
Jayesh BellareDepartment of Chemical Engineering, Indian Institute of Technology Bombay, Mumbai, India.
Kerry HouriganDivision of Biological Engineering, Monash University, Melbourne, Victoria, Australia; Department of Mechanical and Aerospace Engineering, Monash University, Melbourne, Victoria, Australia.
Monash University · AUIndian Institute of Technology Bombay · INSouth Australian Research and Development Institute · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We present detailed quantitative measurement analyses for flow in a spinner flask with spinning rates between 20 to 45 RPM, utilizing the optical velocimetry measurement technique of Particle Image Velocimetry (PIV). A partial section of the impeller was immersed in the working fluid to reduce the shear forces induced on the cells cultured on microcarriers. Higher rotational speeds improved the mixing effect in the medium at the expense of a higher shear environment. It was found that the mouse induced pluripotent stem (iPS) cells achieved the optimum number of cells over 7 days in 25 RPM suspension culture. This condition translates to 0.0984 Pa of maximum shear stress caused by the interaction of the fluid flow with the bottom surface. However, inverse cell growth was obtained at 28 RPM culture condition. Such a narrow margin demonstrated that mouse iPS cells cultured on microcarriers are very sensitive to mechanical forces. This study provides insight to biomechanical parameters, specifically the shear stress distribution, for a commercially available spinner flask over a wide range of Reynolds number.

Indexed as

AnimalsBatch Cell Culture TechniquesBioreactorsCell Culture TechniquesCell ProliferationInduced Pluripotent Stem CellsMiceRheology

Identifiers

PMID25279733
PMCPMC4184809
OpenAlexW1972264416

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.