ArticlePloS one2014
A modified chronic infection model for testing treatment of Staphylococcus aureus biofilms on implants.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 23 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 2 syntheses or guidelines pooled it, 38 citations in OpenAlex.
- Current therapeutic interventions combating biofilm-related infections in orthopaedics : a systematic review of in vivo animal studies.Bone & joint research · 2022Pooled it
- Antibacterial Titanium Implants Biofunctionalized by Plasma Electrolytic Oxidation with Silver, Zinc, and Copper: A Systematic Review.International journal of molecular sciences · 2021Pooled it
- Bithionol is ineffective in a mouse model of S. aureus implant-associated osteomyelitis despite potent in vitro activity.Scientific reports · 2025Article
- Biofilm and the effect of sonication in a chronic Staphylococcus epidermidis orthopedic in vivo implant infection model.Journal of orthopaedic surgery and research · 2024Article
- Extracellular G-quadruplexes and Z-DNA protect biofilms from DNase I, and G-quadruplexes form a DNAzyme with peroxidase activity.Nucleic acids research · 2024Article
- Antibody-Drug Conjugates to Treat Bacterial Biofilms via Targeting and Extracellular Drug Release.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Complete Genome Sequences of Bioluminescent Staphylococcus aureus Strains Xen31 and Xen36, Derived from Two Clinical Isolates.Microbiology resource announcements · 2023Article
- Lugol's solution and Gentian violet eradicate methicillin-resistant Staphylococcus aureus biofilm in skin wound infections.International wound journal · 2023Article
- Fibrinolytic and antibiotic treatment of prosthetic vascular graft infections in a novel rat model.PloS one · 2023Article
- 11C-Para-aminobenzoic acid PET imaging of S. aureus and MRSA infection in preclinical models and humans.JCI insight · 2022Article
- Bacillus subtilis revives conventional antibiotics against Staphylococcus aureus osteomyelitis.Microbial cell factories · 2021Article
- mRNA Transcriptome Analysis of Bone in a Mouse Model of Implant-Associated Staphylococcus aureus Osteomyelitis.Infection and immunity · 2021Article
- Development of a Murine Model of Pyogenic Flexor Tenosynovitis.The Journal of bone and joint surgery. American volume · 2021Article
- Development of systemic immune dysregulation in a rat trauma model of biomaterial-associated infection.Biomaterials · 2021Article
- The role of the msaABCR operon in implant-associated chronic osteomyelitis in Staphylococcus aureus USA300 LAC.BMC microbiology · 2020Article
- Establishment of an in vivo rat model for chronic musculoskeletal implant infection.Journal of orthopaedic surgery and research · 2020Article
- Polylactic-co-glycolic acid microspheres added to fixative cements and its role on bone infected architecture.Journal of biomedical materials research. Part B, Applied biomaterials · 2019Article
- Efficacy of Open Debridement and Polyethylene Exchange in Strictly Selected Patients with Infection after Total Knee Arthroplasty.Knee surgery & related research · 2017Article
- Hyperbaric Oxygen Therapy is Ineffective as an Adjuvant to Daptomycin with Rifampicin Treatment in a Murine Model of Staphylococcus aureus in Implant-Associated Osteomyelitis.Microorganisms · 2017Article
- Early implant-associated osteomyelitis results in a peri-implanted bacterial reservoir.APMIS : acta pathologica, microbiologica, et immunologica Scandinavica · 2017Article
Corrections and comments
- Erratum issuedCorrection: A modified chronic infection model for testing treatment of Staphylococcus aureus biofilms on implants.2015 · Meyer, Rikke [corrected to Meyer, Rikke L]
Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacterial biofilms causing implant-associated osteomyelitis is a severe complication with limited antimicrobial therapy options. We designed an animal model, in which implant associated osteomyelitis arise from a Staphylococcus aureus biofilm on a tibia implant. Two bioluminescently engineered (luxA-E transformed), strains of S. aureus were utilized, Xen29 and Xen31. Biofilm formation was assessed with epifluorescence microscopy. Quantitative measurements were performed day 4, 6, 8, 11 and 15 post-surgery. Bacteria were extracted from the biofilm by sonication and the bacterial load quantified by culturing. Biofilm formation was evident from day 6 post-implantation. Mean bacterial load from implants was ∼1×10(4) CFU/implant, while mean bacterial load from infected tibias were 1×10(6) CFU/bone. Bioluminesence imaging revealed decreasing activity throughout the 15-day observation period, with signal intensity for both strains reaching that of the negative control by day 15 while there was no significant reduction in bacterial load. The model is suitable for testing antimicrobial treatment options for implant associated OM, as treatment efficacy on both biofilm and viable counts can be assessed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.