ArticleFrontiers in neural circuits2014
Simultaneous visualization of extrinsic and intrinsic axon collaterals in Golgi-like detail for mouse corticothalamic and corticocortical cells: a double viral infection method.
Article in Frontiers in neural circuits, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Sleep reveals dynamics integrating and segregating movement and stimulus representations in V1.Nature communications · 2026Article
- Visualization of Selective Neural Pathways by Double Viral Infection Method.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Tracing of specific neural pathways in the rat brain using lentiviral vectors for retrograde gene transfer.Anatomical science international · 2025Article
- Local and long-distance organization of prefrontal cortex circuits in the marmoset brain.Neuron · 2023Article
- The Brain/MINDS Marmoset Connectivity Resource: An open-access platform for cellular-level tracing and tractography in the primate brain.PLoS biology · 2023Article
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- Review
- Transduction of Systemically Administered Adeno-Associated Virus in the Colonic Enteric Nervous System and c-Kit Cells of Adult Mice.Frontiers in neuroanatomy · 2022Article
- Interstitial Axon Collaterals of Callosal Neurons Form Association Projections from the Primary Somatosensory to Motor Cortex in Mice.Cerebral cortex (New York, N.Y. : 1991) · 2021Article
- Application of viral vectors to the study of neural connectivities and neural circuits in the marmoset brain.Developmental neurobiology · 2017Review
- A Mammalian enhancer trap resource for discovering and manipulating neuronal cell types.eLife · 2016Article
- Use of Adeno-Associated and Herpes Simplex Viral Vectors for In Vivo Neuronal Expression in Mice.Current protocols in neuroscience · 2015Article
- Synaptic circuit organization of motor corticothalamic neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
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10 authors.
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Abstract
Here we present a novel tracing technique to stain projection neurons in Golgi-like detail by double viral infection. We used retrograde lentiviral vectors and adeno-associated viral vectors (AAV) to drive "TET-ON/TET-OFF system" in neurons connecting two regions. Using this method, we successfully labeled the corticothalamic (CT) cells of the mouse somatosensory barrel field (S1BF) and motor cortex (M1) in their entirety. We also labeled contra- and ipsilaterally-projecting corticocortical (CC) cells of M1 by targeting contralateral M1 or ipsilateral S1 for retrograde infection. The strength of this method is that we can observe the morphology of specific projection neuron subtypes en masse. We found that the group of CT cells extends their dendrites and intrinsic axons extensively below but not within the thalamorecipient layer in both S1BF and M1, suggesting that the primary target of this cell type is not layer 4. We also found that both ipsi- and contralateral targeting CC cells in M1 commonly exhibit widespread collateral extensions to contralateral M1 (layers 1-6), bilateral S1 and S2 (layers 1, 5 and 6), perirhinal cortex (layers 1, 2/3, 5, and 6), striatum and claustrum. These findings not only strengthened the previous findings of single cell tracings but also extended them by enabling cross-area comparison of CT cells or comparison of CC cells of two different labeling.
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