ArticleWorld journal of gastroenterology2014
Reversible immortalization of human hepatocytes mediated by retroviral transfer and site-specific recombination.
Article in World journal of gastroenterology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Immortalization Reversibility in the Context of Cell Therapy Biosafety.International journal of molecular sciences · 2023Review
- Potential for Isolation of Immortalized Hepatocyte Cell Lines by Liver-DirectedStem cells international · 2019Review
- Reversibly immortalized hepatic progenitor cell line containing double suicide genes.International journal of molecular medicine · 2018Article
- Hepatocyte isolation from resected benign tissues: Results of a 5-year experience.World journal of gastroenterology · 2016Article
- New Tools in Experimental Cellular Therapy for the Treatment of Liver Diseases.Current transplantation reports · 2015Article
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Authors and funding
6 authors.
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Abstract
aimTo establish a method for the reversible immortalization of human hepatocytes, which may offer a good and safe source of hepatocytes for practical applications.
methodsWe successfully isolated primary human hepatocytes from surgically resected liver tissue taken from a patient with liver hemangiomas. The freshly isolated cells were then immortalized with retroviral vector SSR#69 expressing simian virus 40 large T antigen (SV40T) and hygromycin-resistance genes flanked by paired loxP recombination targets.
resultsThe freshly isolated hepatocytes with high viability (85%) were successfully immortalized using retroviral gene transfer of SV40T. SV40T in the immortalized cells was then excised by Cre/loxP site-specific recombination. This cell population exhibited the characteristics of differentiated hepatocytes.
conclusionWe successfully established reversibly immortalized human hepatocytes, which will provide an unlimited supply of cells for practical applications.
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