ArticleOsteoarthritis and cartilage2014
In vivo fluorescence reflectance imaging of protease activity in a mouse model of post-traumatic osteoarthritis.
Article in Osteoarthritis and cartilage, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 1 synthesis or guideline pooled it, 46 citations in OpenAlex.
- An Evidence-Based Systematic Review of Human Knee Post-Traumatic Osteoarthritis (PTOA): Timeline of Clinical Presentation and Disease Markers, Comparison of Knee Joint PTOA Models and Early Disease Implications.International journal of molecular sciences · 2021Pooled it
- Concurrent joint contact in anterior cruciate ligament injury induces cartilage micro-injury and subchondral bone sclerosis, resulting in knee osteoarthritis.Osteoarthritis and cartilage · 2026Article
- Early Unloading After ACL Rupture and Prior to Surgical Restabilization in Mice Slows Post-Traumatic Osteoarthritis Progression.Cartilage · 2026Article
- Cathepsin K Inhibitors as Potential Drugs for the Treatment of Osteoarthritis.International journal of molecular sciences · 2025Review
- Surgical restabilization reduces the progression of post-traumatic osteoarthritis initiated by ACL rupture in mice.Osteoarthritis and cartilage · 2024Article
- A high throughput cell stretch device for investigating mechanobiologyAPL bioengineering · 2024Article
- Validation of a Low-Cost Portable Device for Inducing Noninvasive Anterior Cruciate Ligament Injury in Mice.Journal of biomechanical engineering · 2023Article
- Non-Invasive Compression-Induced Anterior Cruciate Ligament (ACL) Injury and In Vivo Imaging of Protease Activity in Mice.Journal of visualized experiments : JoVE · 2023Article
- Acute Bone Loss and Infrapatellar Fat Pad Fibrosis in the Knee After an In Vivo ACL Injury in Adolescent Mice.The American journal of sports medicine · 2023Article
- Review
- Small-Animal Compression Models of Osteoarthritis.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Article
- New imaging tools for mouse models of osteoarthritis.GeroScience · 2022Review
- Mouse Models of Osteoarthritis: A Summary of Models and Outcomes Assessment.Comparative medicine · 2022Review
- Post-traumatic osteoarthritis progression is diminished by early mechanical unloading and anti-inflammatory treatment in mice.Osteoarthritis and cartilage · 2021Article
- Depth-recognizable time-domain fluorescence molecular tomography in reflective geometry.Biomedical optics express · 2021Article
- Optical Detection of Distal Lung Enzyme Activity in Human Inflammatory Lung Disease.BME frontiers · 2021Article
- Molecular and structural imaging in surgically induced murine osteoarthritis.Osteoarthritis and cartilage · 2020Review
- Recent progress in the imaging detection of enzyme activitiesRSC advances · 2019Review
- Changing landscape of optical imaging in skeletal metastases.Journal of bone oncology · 2019Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
objectiveJoint injuries initiate a surge of inflammatory cytokines and proteases that contribute to cartilage and subchondral bone degeneration. Detecting these early processes in animal models of post-traumatic osteoarthritis (PTOA) typically involves ex vivo analysis of blood serum or synovial fluid biomarkers, or histological analysis of the joint. In this study, we used in vivo fluorescence reflectance imaging (FRI) to quantify protease, matrix metalloproteinase (MMP), and Cathepsin K activity in mice following anterior cruciate ligament (ACL) rupture. We hypothesized that these processes would be elevated at early time points following joint injury, but would return to control levels at later time points.
designMice were injured via tibial compression overload, and FRI was performed at time points from 1 to 56 days after injury using commercially available activatable fluorescent tracers to quantify protease, MMP, and cathepsin K activity in injured vs uninjured knees. PTOA was assessed at 56 days post-injury using micro-computed tomography and whole-joint histology.
resultsProtease activity, MMP activity, and cathepsin K activity were all significantly increased in injured knees relative to uninjured knees at all time points, peaking at 1-7 days post-injury, then decreasing at later time points while still remaining elevated relative to controls.
conclusionsThis study establishes FRI as a reliable method for in vivo quantification of early biological processes in a translatable mouse model of PTOA, and provides crucial information about the time course of inflammation and biological activity following joint injury. These data may inform future studies aimed at targeting these early processes to inhibit PTOA development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.