ArticleMolecular & cellular proteomics : MCP2014
Analysis of differential expression patterns of mRNA and protein during cold-acclimation and de-acclimation in Arabidopsis.
Article in Molecular & cellular proteomics : MCP, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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43 citing papers in PubMed, 81 citations in OpenAlex.
- The CtrCBL1/CtrCIPK6 Complex of Citrus Phosphorylates CtrBBX32 to Regulate CtrSTP1-Mediated Sugar Accumulation and Cold Tolerance.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Plant-pathogen interactions: making the case for multi-omics analysis of complex pathosystems.Stress biology · 2025Review
- WRKY27-Horticulture research · 2025Article
- Multifunctional Transcription Factor YABBY6 Regulates Morphogenesis, Drought and Cold Stress Responses in Rice.Rice (New York, N.Y.) · 2024Article
- Changing turn-over rates regulate abundance of tryptophan, GS biosynthesis, IAA transport and photosynthesis proteins in Arabidopsis growth defense transitions.BMC biology · 2023Article
- Advances in understanding cold tolerance in grapevine.Plant physiology · 2023Review
- Ectopic expression of theFrontiers in plant science · 2023Article
- Proteome and phosphoproteome analysis of 2,4-epibrassinolide-mediated cold stress response in cucumber seedlings.Frontiers in plant science · 2023Article
- Improving abiotic stress tolerance of forage grasses - prospects of using genome editing.Frontiers in plant science · 2023Review
- Article
- Temporal cell wall changes during cold acclimation and deacclimation and their potential involvement in freezing tolerance and growth.Physiologia plantarum · 2023Article
- Glucose-6-P/phosphate translocator2 mediates the phosphoglucose-isomerase1-independent response to microbial volatiles.Plant physiology · 2022Article
- Identification of stably expressed reference genes for expression studies inFrontiers in plant science · 2022Article
- Non-target molecular network and putative genes of flavonoid biosynthesis inFrontiers in plant science · 2022Article
- A combined transcriptomic and proteomic analysis of chrysanthemum provides new insights into petal senescence.Planta · 2021Article
- Comparative physiological and proteomic analyses of mangrove plant Kandelia obovata under cold stress.Ecotoxicology (London, England) · 2021Article
- Evolutionary loss of thermal acclimation accompanied by periodic monocarpic mass flowering in Strobilanthes flexicaulis.Scientific reports · 2021Article
- The AaCBF4-AaBAM3.1 module enhances freezing tolerance of kiwifruit (Actinidia arguta).Horticulture research · 2021Article
- Dynamic modelling of cold-hardiness in tea buds by imitating past temperature memory.Annals of botany · 2021Article
- Identification of the Genetic Basis of Response to De-Acclimation in Winter Barley.International journal of molecular sciences · 2021Article
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Authors and funding
12 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Overwintering plants are capable of exhibiting high levels of cold tolerance, which is acquired through the process of cold acclimation (CA). In contrast to CA, the acquired freezing tolerance is rapidly reduced during cold de-acclimation (DA) and plants resume growth after sensing warm temperatures. In order to better understand plant growth and development, and to aid in the breeding of cold-tolerant plants, it is important to decipher the functional mechanisms of the DA process. In this study, we performed comparative transcriptomic and proteomic analyses during CA and DA. As revealed by shotgun proteomics, we identified 3987 peptides originating from 1569 unique proteins and the corresponding mRNAs were analyzed. Among the 1569 genes, 658 genes were specifically induced at the transcriptional level during the process of cold acclimation. In order to investigate the relationship between mRNA and the corresponding protein expression pattern, a Pearson correlation was analyzed. Interestingly, 199 genes showed a positive correlation of mRNA and protein expression pattern, indicating that both their transcription and translation occurred during CA. However, 226 genes showed a negative correlation of mRNA and protein expression pattern, indicating that their mRNAs were transcribed during CA and were stored for the subsequent DA step. Under this scenario, those proteins were specifically increased during DA without additional transcription of mRNA. In order to confirm the negative correlation of mRNA and protein expression patterns, qRT-PCR and western blot analyses were performed. Mitochondrial malate dehydrogenase 1 (mMDH1) exhibited a negative correlation of mRNA and protein levels, which was characterized by CA-specific mRNA induction and protein accumulation specifically during DA. These data indicate that the expression of specific mRNAs and subsequent accumulation of corresponding proteins are not always in accordance under low temperature stress conditions in plants.
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