ArticleInvestigative ophthalmology & visual science2014
Human limbal mesenchymal cells support the growth of human corneal epithelial stem/progenitor cells.
Article in Investigative ophthalmology & visual science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 54 citations in OpenAlex.
- Human cornea-derived mesenchymal stromal cells inhibit T cells through indoleamine 2,3 dioxygenase.Cytotherapy · 2025Article
- Progress in Transdifferentiation of Autologous Alternative Cell Sources into Corneal Epithelial Cells.Stem cell reviews and reports · 2025Review
- Decoding cellular plasticity and niche regulation of limbal stem cells during corneal wound healing.Stem cell research & therapy · 2024Article
- Clinical Signs of Kawasaki Disease from the Perspective of Epithelial-to-Mesenchymal Transition Recruiting Erythrocytes: A Literature Review.Reviews in cardiovascular medicine · 2023Review
- Article
- Concise Review: Bioengineering of Limbal Stem Cell Niche.Bioengineering (Basel, Switzerland) · 2023Review
- The immunology of corneal limbal stem cells: the up-to-date approach to stem cell transplantation.Central-European journal of immunology · 2023Review
- Review
- Isolation andBio-protocol · 2022Article
- Efficient Isolation and Functional Characterization of Niche Cells from Human Corneal Limbus.International journal of molecular sciences · 2022Article
- Human limbal epithelial stem cell regulation, bioengineering and function.Progress in retinal and eye research · 2021Review
- Limbal stem cell diseases.Experimental eye research · 2021Review
- A Comparison of Methods for Isolation of Limbal Niche Cells: Maintenance of Limbal Epithelial Stem/Progenitor Cells.Investigative ophthalmology & visual science · 2020Article
- Reproducible Derivation and Expansion of Corneal Mesenchymal Stromal Cells for Therapeutic Applications.Translational vision science & technology · 2020Article
- Limbal niche cells can reduce the angiogenic potential of cultivated oral mucosal epithelial cells.Cellular & molecular biology letters · 2019Article
- Rat limbal niche cells can induce transdifferentiation of oral mucosal epithelial cells into corneal epithelial-like cells in vitro.Stem cell research & therapy · 2018Article
- Limbal stromal cells derived from porcine tissue demonstrate mesenchymal characteristics in vitro.Scientific reports · 2017Article
- Article
- A Simple Mechanical Procedure to Create Limbal Stem Cell Deficiency in Mouse.Journal of visualized experiments : JoVE · 2016Article
- Existence of Normal Limbal Epithelium in Eyes With Clinical Signs of Total Limbal Stem Cell Deficiency.Cornea · 2016Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
purposeWe tested the viability of human limbal mesenchymal cells (LMCs) to support the expansion of human corneal epithelial stem/progenitor cells (LSCs).
methodsHuman LMCs were isolated from sclerocorneal tissue using collagenase A. Primary limbal epithelial cells (LECs) in the form of single cell suspension or cell clusters were cocultured on a monolayer of either 3T3 cells (control) or LMCs (SC-LMC culture). The LEC clusters also were grown directly on LMCs (CC-LMC culture) and in an optimized 3-dimensional culture method (3D CC-LMC culture). Colony-forming efficiency (CFE) and LEC proliferation were analyzed. The phenotype of the cultured LECs was assessed by their expression level of putative stem cell markers and a differentiation marker by qRT-PCR and immunocytochemistry.
resultsThe LECs in the SC-LMC culture had a very limited growth and the stem/progenitor phenotype was lost compared to the control. Growth and cell morphology improved using the CC-LMC culture. The 3D CC-LMC culture method was the best to support the growth of the LSC population. Expression of ATP-binding cassette family G2 and ΔNp63 at the mRNA level was maintained or increased in CC-LMCs and 3D CC-LMC cultures compared to the control. The percentage of the K14(+) and K12(+) cells was comparable in these three cultures. There was no significant difference in the percentage of p63α high expressing cells in the control (21%) and 3D CC-LMC culture (17%, P > 0.05).
conclusionsHuman LMCs can substitute 3T3 cells in the expansion of LSCs using the 3-dimensional culture system.
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