ArticleTissue engineering. Part C, Methods2015
Live fibroblast harvest reveals surface marker shift in vitro.
Article in Tissue engineering. Part C, Methods, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 33 citations in OpenAlex.
- Stromal heterogeneity in the adult lung delineated by single-cell genomics.American journal of physiology. Cell physiology · 2025Review
- Characterizing Fibroblast Heterogeneity in Diabetic Wounds Through Single-Cell RNA-Sequencing.Biomedicines · 2024Review
- Characterization and Physiological Differences of Two Primary Cultures of Human Normal and Hypertrophic Scar Dermal Fibroblasts: A Pilot Study.Biomedicines · 2024Article
- Understanding Tendon Fibroblast Biology and Heterogeneity.Biomedicines · 2024Review
- Autocrine IL-6 drives cell and extracellular matrix anisotropy in scar fibroblasts.Matrix biology : journal of the International Society for Matrix Biology · 2023Article
- Article
- Distinct fibroblast progenitor subpopulation expedites regenerative mucosal healing by immunomodulation.The Journal of experimental medicine · 2023Article
- Polycomb Bodies Detection in Murine Fibromuscular Stroma from Skin, Skeletal Muscles, and Aortic Tissues.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Wound healing, fibroblast heterogeneity, and fibrosis.Cell stem cell · 2022Review
- An Optimized Tissue Dissociation Protocol for Single-Cell RNA Sequencing Analysis of Fresh and Cultured Human Skin Biopsies.Frontiers in cell and developmental biology · 2022Article
- Diabetic wound healing in soft and hard oral tissues.Translational research : the journal of laboratory and clinical medicine · 2021Review
- A developmental basis for the anatomical diversity of dermis in homeostasis and wound repair.The Journal of pathology · 2021Article
- Dissecting Fibroblast Heterogeneity in Health and Fibrotic Disease.Current rheumatology reports · 2020Review
- Fibroblast Heterogeneity in and Its Implications for Plastic and Reconstructive Surgery: A Basic Science Review.Plastic and reconstructive surgery. Global open · 2020Article
- Small molecule inhibition of dipeptidyl peptidase-4 enhances bone marrow progenitor cell function and angiogenesis in diabetic wounds.Translational research : the journal of laboratory and clinical medicine · 2019Article
- In Vitro and In Vivo Osteogenic Differentiation of Human Adipose-Derived Stromal Cells.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Connexin 43 regulates the expression of wound healing-related genes in human gingival and skin fibroblasts.Experimental cell research · 2018Article
- Fibroblast heterogeneity: implications for human disease.The Journal of clinical investigation · 2018Review
- Isolating and Analyzing Cells of the Pancreas Mesenchyme by Flow Cytometry.Journal of visualized experiments : JoVE · 2017Article
- Altered Dermal Fibroblasts in Systemic Sclerosis Display Podoplanin and CD90.The American journal of pathology · 2016Article
Corrections and comments
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Authors and funding
14 authors at 1 institution in 1 country.
Funding
Abstract
Current methods for the isolation of fibroblasts require extended ex vivo manipulation in cell culture. As a consequence, prior studies investigating fibroblast biology may fail to adequately represent cellular phenotypes in vivo. To overcome this problem, we describe a detailed protocol for the isolation of fibroblasts from the dorsal dermis of adult mice that bypasses the need for cell culture, thereby preserving the physiological, transcriptional, and proteomic profiles of each cell. Using the described protocol we characterized the transcriptional programs and the surface expression of 176 CD markers in cultured versus uncultured fibroblasts. The differential expression patterns we observed highlight the importance of a live harvest for investigations of fibroblast biology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.