ArticlePLoS genetics2014
Evidence of a bacterial receptor for lysozyme: binding of lysozyme to the anti-σ factor RsiV controls activation of the ecf σ factor σV.
Article in PLoS genetics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 42 citations in OpenAlex.
- Mixed positive and negative feedback loops drive diverse single-cell gene expression dynamics.Nature communications · 2026Article
- Identification of DraRS inJournal of bacteriology · 2023Article
- A Decrease in Fatty Acid Synthesis Rescues Cells with Limited Peptidoglycan Synthesis Capacity.mBio · 2023Article
- The role of site-2-proteases in bacteria: a review on physiology, virulence, and therapeutic potential.microLife · 2023Review
- Disruption of theJournal of bacteriology · 2022Article
- The Cationic Antimicrobial Peptide Activity of Lysozyme Reduces ViableAntimicrobial agents and chemotherapy · 2022Article
- Activation of the Extracytoplasmic Function σ Factor σmSphere · 2022Article
- Activation of the extracytoplasmic function σ factor σCurrent opinion in microbiology · 2022Review
- SigV Mediates Lysozyme Resistance in Enterococcus faecalis via RsiV and PgdA.Journal of bacteriology · 2021Article
- Tunable phenotypic variability through an autoregulatory alternative sigma factor circuit.Molecular systems biology · 2021Article
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- Lysozyme Resistance in Clostridioides difficile Is Dependent on Two Peptidoglycan Deacetylases.Journal of bacteriology · 2020Article
- Effect of Lipidation on the Localization and Activity of a Lysozyme Inhibitor inJournal of bacteriology · 2020Article
- Activation of the Extracytoplasmic Function σ Factor σmSphere · 2019Article
- Defining the regulon of genes controlled by σMolecular microbiology · 2019Article
- Activation of the extracytoplasmic function σ factor σMolecular microbiology · 2019Review
- The crystal structure of Mycobacterium tuberculosis high-temperature requirement A protein reveals an autoregulatory mechanism.Acta crystallographica. Section F, Structural biology communications · 2018Article
- Bacterial sensing: A putative amphipathic helix in RsiV is the switch for activating σV in response to lysozyme.PLoS genetics · 2018Article
- Neisseria gonorrhoeae employs two protein inhibitors to evade killing by human lysozyme.PLoS pathogens · 2018Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
σ factors endow RNA polymerase with promoter specificity in bacteria. Extra-Cytoplasmic Function (ECF) σ factors represent the largest and most diverse family of σ factors. Most ECF σ factors must be activated in response to an external signal. One mechanism of activation is the stepwise proteolytic destruction of an anti-σ factor via Regulated Intramembrane Proteolysis (RIP). In most cases, the site-1 protease required to initiate the RIP process directly senses the signal. Here we report a new mechanism in which the anti-σ factor rather than the site-1 protease is the sensor. We provide evidence suggesting that the anti-σ factor RsiV is the bacterial receptor for the innate immune defense enzyme, lysozyme. The site-1 cleavage site is similar to the recognition site of signal peptidase and cleavage at this site is required for σV activation in Bacillus subtilis. We reconstitute site-1 cleavage in vitro and demonstrate that it requires both signal peptidase and lysozyme. We demonstrate that the anti-σ factor RsiV directly binds to lysozyme and muramidase activity is not required for σV activation. We propose a model in which the binding of lysozyme to RsiV activates RsiV for signal peptidase cleavage at site-1, initiating proteolytic destruction of RsiV and activation of σV. This suggests a novel mechanism in which conformational change in a substrate controls the cleavage susceptibility for signal peptidase. Thus, unlike other ECF σ factors which require regulated intramembrane proteolysis for activation, the sensor for σV activation is not the site-1 protease but the anti-σ factor.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.