ArticlePloS one2014
Hybridization capture using short PCR products enriches small genomes by capturing flanking sequences (CapFlank).
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed.
- Methods, applications, and computational challenges in bait capture enrichment.Cell reports methods · 2025Review
- The complete mitochondrial genome of Poncelet's giant rat (Mitochondrial DNA. Part B, Resources · 2025Article
- ORF Capture-Seq as a versatile method for targeted identification of full-length isoforms.Nature communications · 2020Article
- Review
- Impact of Interferon-α Receptor-1 Promoter Polymorphisms on the Transcriptome of the Hepatitis B Virus-Associated Hepatocellular Carcinoma.Frontiers in immunology · 2018Article
- Clinical and biological insights from viral genome sequencing.Nature reviews. Microbiology · 2017Review
- Article
- Phylogeography of the Koala, (Phascolarctos cinereus), and Harmonising Data to Inform Conservation.PloS one · 2016Article
- Article
- Hybridization Capture Using RAD Probes (hyRAD), a New Tool for Performing Genomic Analyses on Collection Specimens.PloS one · 2016Article
- A novel method for the multiplexed target enrichment of MinION next generation sequencing libraries using PCR-generated baits.Nucleic acids research · 2015Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Solution hybridization capture methods utilize biotinylated oligonucleotides as baits to enrich homologous sequences from next generation sequencing (NGS) libraries. Coupled with NGS, the method generates kilo to gigabases of high confidence consensus targeted sequence. However, in many experiments, a non-negligible fraction of the resulting sequence reads are not homologous to the bait. We demonstrate that during capture, the bait-hybridized library molecules add additional flanking library sequences iteratively, such that baits limited to targeting relatively short regions (e.g. few hundred nucleotides) can result in enrichment across entire mitochondrial and bacterial genomes. Our findings suggest that some of the off-target sequences derived in capture experiments are non-randomly enriched, and that CapFlank will facilitate targeted enrichment of large contiguous sequences with minimal prior target sequence information.
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Registered trials
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