ArticlePloS one2014
Site-specific chemoenzymatic labeling of aerolysin enables the identification of new aerolysin receptors.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 56 citations in OpenAlex.
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- Repurposing a plant peptide cyclase for targeted lysine acylation.Nature chemistry · 2024Article
- Article
- Combined Application of Orthogonal Sortases and Depsipeptide Substrates for Dual Protein Labeling.Bioconjugate chemistry · 2022Article
- Palmatine Inhibits the Pathogenicity ofFoods (Basel, Switzerland) · 2022Article
- Genomic surveillance reveals antibiotic resistance gene transmission via phage recombinases within sheep mastitis-associated Streptococcus uberis.BMC veterinary research · 2022Article
- Challenges in the use of sortase and other peptide ligases for site-specific protein modification.Chemical Society reviews · 2022Review
- Synthesis of cholera toxin B subunit glycoconjugates using site-specific orthogonal oxime and sortase ligation reactions.Frontiers in chemistry · 2022Article
- Luteolin decreases the pathogenicity ofVirulence · 2021Article
- Engineered Sortases in Peptide and Protein Chemistry.Chembiochem : a European journal of chemical biology · 2021Review
- Sequence characterisation and novel insights into bovine mastitis-associated Streptococcus uberis in dairy herds.Scientific reports · 2021Article
- Sortase-mediated segmental labeling: A method for segmental assignment of intrinsically disordered regions in proteins.PloS one · 2021Article
- Genistein Inhibits the Pathogenesis ofFrontiers in pharmacology · 2021Article
- Chemoenzymatic Semisynthesis of Proteins.Chemical reviews · 2020Review
- Site-Specific Protein Labeling via Sortase-Mediated Transpeptidation.Current protocols in protein science · 2017Article
- Rapid and Direct VHH and Target Identification by Staphylococcal Surface Display Libraries.International journal of molecular sciences · 2017Article
- Targeting the N terminus for site-selective protein modification.Nature chemical biology · 2017Review
- Generation of Ca2+-independent sortase A mutants with enhanced activity for protein and cell surface labeling.PloS one · 2017Article
- A molecular engineering toolbox for the structural biologist.Quarterly reviews of biophysics · 2017Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Aerolysin is a secreted bacterial toxin that perforates the plasma membrane of a target cell with lethal consequences. Previously explored native and epitope-tagged forms of the toxin do not allow site-specific modification of the mature toxin with a probe of choice. We explore sortase-mediated transpeptidation reactions (sortagging) to install fluorophores and biotin at three distinct sites in aerolysin, without impairing binding of the toxin to the cell membrane and with minimal impact on toxicity. Using a version of aerolysin labeled with different fluorophores at two distinct sites we followed the fate of the C-terminal peptide independently from the N-terminal part of the toxin, and show its loss in the course of intoxication. Making use of the biotinylated version of aerolysin, we identify mesothelin, urokinase plasminogen activator surface receptor (uPAR, CD87), glypican-1, and CD59 glycoprotein as aerolysin receptors, all predicted or known to be modified with a glycosylphosphatidylinositol anchor. The sortase-mediated reactions reported here can be readily extended to other pore forming proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.