ArticlePLoS pathogens2014
Insights into Vibrio cholerae intestinal colonization from monitoring fluorescently labeled bacteria.
Article in PLoS pathogens, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 126 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
126 citing papers in PubMed, 194 citations in OpenAlex.
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- Vibrio cholerae-synthesized tryptophan activates innate immune signaling in enteroendocrine cells via phosphorylation of the nuclear receptor ultraspiracle.Nature communications · 2026Article
- The role of cytochrome oxidases in bacterial virulence.Infection and immunity · 2026Review
- Environmental regulation of virulence in Vibrio cholerae: integrating multi-omics, predictive models, and comparative insights across emerging Vibrio species.Gut pathogens · 2026Review
- IL-22 promotes genesis of small intestinal secretory cells that protect against cholera in mice.Nature microbiology · 2026Article
- Interspecies quorum sensing signals modulate multicellular organization and enhance contact-dependent antagonism in Vibrio cholerae.Nature communications · 2026Article
- ICP1 bacteriophage treatment antagonizes colonization of the zebrafish larval intestine byMicrobiology spectrum · 2026Article
- IL-22 promotes genesis of small intestinal secretory cells that protect against cholera in mice.bioRxiv : the preprint server for biology · 2026Article
- Modular small RNA drives the emergence of virulence traits and environmental trade-offs in Vibrio cholerae.Nature communications · 2026Article
- RND-mediated efflux couples antimicrobial resistance and hypervirulence in contemporary Vibrio cholerae.PLoS pathogens · 2026Article
- A quorum-sensing molecule fromProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Cell-body curvature modulates stall frequency to enhancebioRxiv : the preprint server for biology · 2026Article
- DPO production by gut commensals suggests a broader role in bacterial communication and host-microbiome interactions.NPJ biofilms and microbiomes · 2025Article
- Human antibody targetingmBio · 2025Article
- Vibrio cholerae motility is associated with inter-animal transmission.Nature communications · 2025Article
- Navigating bacterial motility through chemotaxis: from molecular mechanisms to physiological perspectives.Folia microbiologica · 2025Review
- Allelic variations and gene cluster modularity act as nonlinear bottlenecks for cholera emergence.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Orally delivered toxin-binding protein protects against diarrhoea in a murine cholera model.Nature communications · 2025Article
- ProQ-associated small RNAs control motility in Vibrio cholerae.Nucleic acids research · 2025Article
- The role of morphological adaptability inmBio · 2025Article
66 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Vibrio cholerae, the agent of cholera, is a motile non-invasive pathogen that colonizes the small intestine (SI). Most of our knowledge of the processes required for V. cholerae intestinal colonization is derived from enumeration of wt and mutant V. cholerae recovered from orogastrically infected infant mice. There is limited knowledge of the distribution of V. cholerae within the SI, particularly its localization along the villous axis, or of the bacterial and host factors that account for this distribution. Here, using confocal and intravital two-photon microscopy to monitor the localization of fluorescently tagged V. cholerae strains, we uncovered unexpected and previously unrecognized features of V. cholerae intestinal colonization. Direct visualization of the pathogen within the intestine revealed that the majority of V. cholerae microcolonies attached to the intestinal epithelium arise from single cells, and that there are notable regiospecific aspects to V. cholerae localization and factors required for colonization. In the proximal SI, V. cholerae reside exclusively within the developing intestinal crypts, but they are not restricted to the crypts in the more distal SI. Unexpectedly, V. cholerae motility proved to be a regiospecific colonization factor that is critical for colonization of the proximal, but not the distal, SI. Furthermore, neither motility nor chemotaxis were required for proper V. cholerae distribution along the villous axis or in crypts, suggesting that yet undefined processes enable the pathogen to find its niches outside the intestinal lumen. Finally, our observations suggest that host mucins are a key factor limiting V. cholerae intestinal colonization, particularly in the proximal SI where there appears to be a more abundant mucus layer. Collectively, our findings demonstrate the potent capacity of direct pathogen visualization during infection to deepen our understanding of host pathogen interactions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.