ArticlePloS one2014
The largest subunit of RNA polymerase II as a new marker gene to study assemblages of arbuscular mycorrhizal fungi in the field.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 71 citations in OpenAlex.
- Article
- Community composition of arbuscular mycorrhizal fungi in Ontario tallgrass prairies of differing disturbance histories.Mycorrhiza · 2026Article
- Molecular markers for the study of arbuscular mycorrhizal fungi.Archives of microbiology · 2026Review
- A new genus (MycoKeys · 2026Article
- The pitfalls of rDNA-based AMF identification: a comparative analysis of rDNA and protein-coding genes.The New phytologist · 2025Article
- Comparative Mitogenome Analysis ofJournal of fungi (Basel, Switzerland) · 2025Article
- Protein Coding Low-CopyEcology and evolution · 2025Article
- Synonymization of three species of Rhizophagus based on morphological and molecular evidence and biogeography of Rhizophagus clarus.Mycorrhiza · 2025Article
- Molecular phylogeny, morphology, mycorrhizal symbiosis, and putative distribution of the arbuscular mycorrhizal fungusMycoscience · 2025Article
- MycoKeys · 2025Article
- Shift in the rhizosphere soil fungal community associated with root rot infection of Plukenetia volubilis Linneo caused by Fusarium and Rhizopus species.International microbiology : the official journal of the Spanish Society for Microbiology · 2024Article
- Article
- Three new species of arbuscular mycorrhizal fungi (Glomeromycota) andFrontiers in microbiology · 2024Article
- Isolation and identification of an arbuscular mycorrhizal fungus specifically associated with mycoheterotrophic seedlings ofMycoscience · 2023Article
- Illumina MiSeq Sequencing Reveals Correlations among Fruit Ingredients, Environmental Factors, and AMF Communities in ThreeMicrobiology spectrum · 2022Article
- A new order, Entrophosporales, and three newFrontiers in microbiology · 2022Article
- A historical perspective on mycorrhizal mutualism emphasizing arbuscular mycorrhizas and their emerging challenges.Mycorrhiza · 2021Review
- New Glomeromycotan Taxa,Frontiers in microbiology · 2021Article
- Resolving the Mortierellaceae phylogeny through synthesis of multi-gene phylogenetics and phylogenomics.Fungal diversity · 2020Article
- Insights into the complex role of GRAS transcription factors in the arbuscular mycorrhiza symbiosis.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Due to the potential of arbuscular mycorrhizal fungi (AMF, Glomeromycota) to improve plant growth and soil quality, the influence of agricultural practice on their diversity continues to be an important research question. Up to now studies of community diversity in AMF have exclusively been based on nuclear ribosomal gene regions, which in AMF show high intra-organism polymorphism, seriously complicating interpretation of these data. We designed specific PCR primers for 454 sequencing of a region of the largest subunit of RNA polymerase II gene, and established a new reference dataset comprising all major AMF lineages. This gene is known to be monomorphic within fungal isolates but shows an excellent barcode gap between species. We designed a primer set to amplify all known lineages of AMF and demonstrated its applicability in combination with high-throughput sequencing in a long-term tillage experiment. The PCR primers showed a specificity of 99.94% for glomeromycotan sequences. We found evidence of significant shifts of the AMF communities caused by soil management and showed that tillage effects on different AMF taxa are clearly more complex than previously thought. The high resolving power of high-throughput sequencing highlights the need for quantitative measurements to efficiently detect these effects.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.