Evidence map›Paper›PMID 25275114›Full record

ReviewCold Spring Harbor protocols2014

Fluorescence microscopy.

Michael J Sanderson, Ian Smith, Ian Parker, Martin D Bootman

Open access · bronzeAbstract readReview
In one paragraph

Review in Cold Spring Harbor protocols, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 141 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
141citing papers in PubMed, 2 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

141 citing papers in PubMed, 2 syntheses or guidelines pooled it, 334 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Article
  4. Article
  5. Review
  6. Observational
  7. Review
  8. Monitoring Gene Expression in Retina with synthetic serum markers.bioRxiv : the preprint server for biology · 2026
    Article
  9. Article
  10. Review
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Article

81 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 3 institutions in 2 countries.

Michael J SandersonDepartment of Microbiology and Physiology Systems, University of Massachusetts Medical School, Worcester, Massachusetts 01655;
Ian SmithDepartment of Neurobiology & Behavior, University of California, Irvine, California 92697-4550;
Ian ParkerDepartment of Neurobiology & Behavior, University of California, Irvine, California 92697-4550;
Martin D BootmanDepartment of Life, Health and Chemical Sciences, The Open University, Milton Keynes MK7 6AA, United Kingdom.
University of California, Irvine · USThe Open University · GBUniversity of Massachusetts Chan Medical School · US

Funding

Elementary Events of Intracellular Calcium SignalingR37GM048071 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI PARKER, IAN · 2011 to 2020
$5.3M
SPATIAL AND TEMPORAL ASPECTS OF INSP3 SIGNALLINGR01GM048071 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI PARKER, IAN · 1992 to 2010
$3.9M
A multi-scale approach to airway hyperresponsiveness: from molecule to organR01HL103405 · NHLBI · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI BATES, JASON HT, LAUZON, ANNE-MARIE · 2010 to 2013
$3.6M
Calcium Signals Within Membrane NanotubesR01GM100201 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI SMITH, IAN F · 2012 to 2016
$1.5M
NHLBI NIH HHS HL103405NHLBI NIH HHS R01 HL103405NIGMS NIH HHS GM048071NIGMS NIH HHS GM100201NIGMS NIH HHS R01 GM048071NIGMS NIH HHS R01 GM100201NIGMS NIH HHS R37 GM048071
6 · The paper itself

Abstract

Fluorescence microscopy is a major tool with which to monitor cell physiology. Although the concepts of fluorescence and its optical separation using filters remain similar, microscope design varies with the aim of increasing image contrast and spatial resolution. The basics of wide-field microscopy are outlined to emphasize the selection, advantages, and correct use of laser scanning confocal microscopy, two-photon microscopy, scanning disk confocal microscopy, total internal reflection, and super-resolution microscopy. In addition, the principles of how these microscopes form images are reviewed to appreciate their capabilities, limitations, and constraints for operation.

Indexed as

AnimalsFluorescenceHumansMicroscopy, FluorescenceOptical Devices

Identifiers

PMID25275114
PMCPMC4711767
OpenAlexW4233083522

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.