ArticleJournal of clinical microbiology2014
Efficient diagnosis and treatment follow-up of human brucellosis by a novel quantitative TaqMan real-time PCR assay: a human clinical survey.
Article in Journal of clinical microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 30 citations in OpenAlex.
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- New area of occurrence of human brucellosis in Brazil: serological and molecular prevalence and risk factors associated with Brucella abortus infection.One health outlook · 2025Article
- Biosensor Technology: Advances and Applications in Livestock Infectious Disease Diagnosis.Veterinary sciences · 2025Review
- Brucellosis in the Brazilian Pantanal wetland: threat to animal production and wildlife conservation.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2022Review
- Increased serum piwi-interacting RNAs as a novel potential diagnostic tool for brucellosis.Frontiers in cellular and infection microbiology · 2022Article
- Optimization and validation of a real-time polymerase chain reaction protocol for the diagnosis of human brucellosis.Folia microbiologica · 2020Article
- Laboratory Diagnosis of Human Brucellosis.Clinical microbiology reviews · 2019Review
- Outbreak of human brucellosis in Southern Brazil and historical review of data from 2009 to 2018.PLoS neglected tropical diseases · 2018Article
- Comparing Rapid and Specific Detection of Brucella in Clinical Samples by PCR-ELISA and Multiplex-PCR Method.Iranian journal of pathology · 2016Article
- MicroRNA-410-5p as a potential serum biomarker for the diagnosis of prostate cancer.Cancer cell international · 2016Article
- Lymphadenopathy and fever in a chef during a stay in Europe.Jornal brasileiro de pneumologia : publicacao oficial da Sociedade Brasileira de Pneumologia e TisilogiaReview
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rapid and effective diagnosis of brucellosis is a challenge for clinicians. Even when diagnosis is on time and therapy is initiated, meticulous follow-up appointments are crucial for ensuring the efficacy of the treatment. Due to shortcomings of serological methods, molecular diagnosis, especially real-time PCR, is becoming a main approach in laboratory diagnostics. Thus, the development of efficient procedures and standardization of the PCR tests will have a great impact on the precise detection and quantification of bacterial DNA loads, which is valuable for the medical management of brucellosis patients. We developed a new TaqMan real-time PCR directed to bcsp31, a shared gene of the brucellae. The bcsp31 gene fragment was cloned into pJET1.2. Recombinant pJET1.2-bcsp31 was linearized by HindIII digestion, and the product was used for the preparation of a standard curve. A panel of Brucella spp. and non-Brucella pathogens was tested. No bacterial genomes other than those of the brucellae were detected. According to the results, specificity of the method was 100%. In a clinical assessment, the positive-control group comprised 37 patients with microbiologically confirmed brucellosis, and 25 healthy individuals served as the negative-control group. By the end of the treatment period, there was a significant decrease in the DNA load of the 37 brucellosis patients, which persisted for the 4 weeks of monitoring after treatment, suggesting that our proposed method is an efficient monitoring tool. Serum samples prior to any treatment were collected from the 25 serologically suspicious patients and assessed by our method; 72% of these patients tested positive for brucellosis.
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