ArticleJournal of lipid research2014
Quantitative imaging mass spectrometry of renal sulfatides: validation by classical mass spectrometric methods.
Article in Journal of lipid research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 34 citations in OpenAlex.
- Review
- Deep MALDI-MS spatial omics guided by quantum cascade laser mid-infrared imaging microscopy.Nature communications · 2025Article
- Sulfatide with ceramide composed of phytosphingosine (t18:0) and 2-hydroxy FAs in renal intercalated cells.Journal of lipid research · 2022Article
- Sulfatides are endogenous ligands for the TLR4-MD-2 complex.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- High-Resolution Human Kidney Molecular Histology by Imaging Mass Spectrometry of Lipids.Analytical chemistry · 2021Article
- Recent advances in the mass spectrometric analysis of glycosphingolipidome - A review.Analytica chimica acta · 2020Review
- Kidney Lipidomics by Mass Spectrometry Imaging: A Focus on the Glomerulus.International journal of molecular sciences · 2019Review
- Defining Changes in the Spatial Distribution and Composition of Brain Lipids in the Shiverer and Cuprizone Mouse Models of Myelin Disease.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2019Article
- Recent Developments of Useful MALDI Matrices for the Mass Spectrometric Characterization of Lipids.Biomolecules · 2018Review
- Mass spectrometry imaging of lipids: untargeted consensus spectra reveal spatial distributions in Niemann-Pick disease type C1.Journal of lipid research · 2018Article
- MALDI Orbitrap Mass Spectrometry Profiling of Dysregulated Sulfoglycosphingolipids in Renal Cell Carcinoma Tissues.Journal of the American Society for Mass Spectrometry · 2017Article
- Mass Spectrometry Imaging proves differential absorption profiles of well-characterised permeability markers along the crypt-villus axis.Scientific reports · 2017Article
- Renal sulfatides: sphingoid base-dependent localization and region-specific compensation of CerS2-dysfunction.Journal of lipid research · 2014Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Owing to its capability of discriminating subtle mass-altering structural differences such as double bonds or elongated acyl chains, MALDI-based imaging MS (IMS) has emerged as a powerful technique for analysis of lipid distribution in tissue at moderate spatial resolution of about 50 μm. However, it is still unknown if MS(1)-signals and ion intensity images correlate with the corresponding apparent lipid concentrations. Analyzing renal sulfated glycosphingolipids, sulfatides, we validate for the first time IMS-signal identities using corresponding sulfatide-deficient kidneys. To evaluate the extent of signal quenching effects interfering with lipid quantification, we surgically dissected the three major renal regions (papillae, medulla, and cortex) and systematically compared MALDI IMS of renal sulfatides with quantitative analyses of corresponding lipid extracts by on-target MALDI TOF-MS and by ultra-performance LC-ESI-(triple-quadrupole)tandem MS. Our results demonstrate a generally strong correlation (R(2) > 0.9) between the local relative sulfatide signal intensity in MALDI IMS and absolute sulfatide quantities determined by the other two methods. However, high concentrations of sulfatides in the papillae and medulla result in an up to 4-fold signal suppression. In conclusion, our study suggests that MALDI IMS is useful for semi-quantitative dissection of relative local changes of sulfatides and possibly other lipids in tissue.
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