ArticleParasites & vectors2014
Suitability of internal transcribed spacers (ITS) as markers for the population genetic structure of Blastocystis spp.
Article in Parasites & vectors, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed.
- Aspects of Genetic Diversity, Host Specificity and Public Health Significance of Single-Celled Intestinal Parasites Commonly Observed in Humans and Mostly Referred to as 'Non-Pathogenic'.APMIS : acta pathologica, microbiologica, et immunologica Scandinavica · 2025Review
- Molecular Identification ofJournal of clinical medicine · 2025Article
- Genetic Variation among the Partial Gene Sequences of the Ribosomal Protein Large-Two, the Internal Transcribed Spacer, and the Small Ribosomal Subunit ofMicroorganisms · 2024Article
- Subtype-host patterns and genetic differentiation ofHeliyon · 2024Article
- Genetic Variability of the Internal Transcribed Spacer and Pyruvate:Ferredoxin Oxidoreductase Partial Gene ofMicroorganisms · 2023Article
- An update on the distribution ofHeliyon · 2022Article
- Identification of α-L-fucosidase (ALFuc) of Blastocystis sp. subtypes ST1, ST2 and ST3.Revista do Instituto de Medicina Tropical de Sao Paulo · 2022Article
- Identification of MultipleFrontiers in veterinary science · 2021Article
- Succinate dehydrogenase gene as a marker for studyingHeliyon · 2020Article
- An Unusual Case of Extra-Enteric Blastocystosis in the Uterine Cervix.The Korean journal of parasitology · 2020Article
- Subtypes ofParasite epidemiology and control · 2020Article
- Molecular genotyping of Blastocystis spp. in wild mammals from Mexico.Parasitology research · 2020Article
- A summary of Blastocystis subtypes in North and South America.Parasites & vectors · 2019Review
- Can the pyruvate: ferredoxin oxidoreductase (PFOR) gene be used as an additional marker to discriminate among Blastocystis strains or subtypes?Parasites & vectors · 2018Article
- Genetic Diversity and Distribution ofBioMed research international · 2018Article
- Large-scale comparative metagenomics of Blastocystis, a common member of the human gut microbiome.The ISME journal · 2017Article
- New specific primers for amplification of the Internal Transcribed Spacer region in Clitellata (Annelida).Ecology and evolution · 2017Article
- Clarifying the Cryptic Host Specificity of Blastocystis spp. Isolates from Alouatta palliata and A. pigra Howler Monkeys.PloS one · 2017Article
- Population expansion and gene flow in Giardia duodenalis as revealed by triosephosphate isomerase gene.Parasites & vectors · 2015Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe purpose of this study was to assess the genetic variation and differentiation of Blastocystis subtypes (STs) recovered from symptomatic children by analysing partial sequences of the small subunit rDNA gene region (SSUrDNA) and internal transcribed spacers (1 and 2) plus the 5.8S region (ITS, ITS1 + 5.8S + ITS2) and comparing with isolates from other countries.
findingsFaecal samples from 47 Blastocystis-infected children with gastrointestinal symptoms and negative for pathogenic enterobacteria were analysed. PCR was performed on DNA from all the samples to identify Blastocystis STs, amplifying a fragment of SSUrDNA and the ITS region. The amplicons were purified and sequenced, and consensus sequences were submitted to GenBank; afterwards, SSUrDNA sequences were analysed for genetic diversity according to geographic area. Regarding the Blastocystis STs found, 51% were ST1, 23% ST2, 19% ST3 and 2% ST7. For ITS, a haplotype network tree and Bayesian inference revealed the presence of two novel variants of ST1, clustering some sequences into ST1A and ST1B. The values of nucleotide diversity (π) and haplotype polymorphism (θ) for ST1, ST2 and ST3 ranged from 0 to 1, whereas the ratio of genetic differentiation (FST)/migration index (Nm) showed the highest differentiation between Libya and Thailand-Philippines for ST2 (0.282/0.63). In contrast, a high flow gene was observed between Czech Republic-Denmark-Holland-Spain and USA-Mexico-Colombia for ST1 (0.003/84).
conclusionOur data on genetic differentiation and gene flow might explain the differences for the prevalence of Blastocystis STs. Moreover, the ITS region could be used as a genetic marker to assess genetic variation in this parasite.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.