ArticleParasites & vectors2014
Comparison of PCR methods for detection of Leishmania siamensis infection.
Article in Parasites & vectors, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed, 43 citations in OpenAlex.
- Cutaneous Leishmaniasis in Southeastern Algeria: Clinical, Parasitological Diagnosis and Molecular Identification.Acta parasitologica · 2025Article
- Incidence and persistence of asymptomatic Leishmania infection among HIV-infected patients in Trang province, Southern Thailand: A cohort study.PLoS neglected tropical diseases · 2024Article
- Nanopore-Based Surveillance ofInsects · 2024Article
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- Novel duplex TaqMan-based quantitative PCR for rapid and accurate diagnosis ofCurrent research in parasitology & vector-borne diseases · 2024Article
- Genetic variation and geographic distribution of Leishmania orientalis and Leishmania martiniquensis among Leishmania/HIV co-infection in Thailand.Scientific reports · 2023Article
- Leishmania donovani persistence and circulation causing cutaneous leishmaniasis in unusual-foci of Nepal.Scientific reports · 2023Article
- Detection of Leishmania donovani using ITS1-RFLP from positive and negative smear samples among clinically reported patients visiting University of Gondar Comprehensive Specialized Hospital.BMC infectious diseases · 2022Article
- Laboratory diagnostics for human Leishmania infections: a polymerase chain reaction-focussed review of detection and identification methods.Parasites & vectors · 2022Review
- Concurrent molecular characterization of sand flies and Leishmania parasites by amplicon-based next-generation sequencing.Parasites & vectors · 2022Article
- Development of a nested PCR assay for specific detection ofFrontiers in cellular and infection microbiology · 2022Article
- Molecular identification of two newly identified human pathogens causing leishmaniasis using PCR-based methods on the 3' untranslated region of the heat shock protein 70 (type I) gene.PLoS neglected tropical diseases · 2021Article
- Evaluation of conventional and four real-time PCR methods for the detection of Leishmania on field-collected samples in Ethiopia.PLoS neglected tropical diseases · 2021Article
- Visceral Leishmaniasis in Traveler to Guyana Caused by Leishmania siamensis, London, UK.Emerging infectious diseases · 2018Article
- Prevalence and risk factors associated with Leishmania infection in Trang Province, southern Thailand.PLoS neglected tropical diseases · 2017Article
- Comparison of LAMP and PCR for molecular mass screening of sand flies for Leishmania martiniquensis infection.Memorias do Instituto Oswaldo Cruz · 2017Article
- Analytical Performance of Four Polymerase Chain Reaction (PCR) and Real Time PCR (qPCR) Assays for the Detection of SixFrontiers in microbiology · 2017Article
- Assessment of the sensitivity and specificity of serological (IFAT) and molecular (direct-PCR) techniques for diagnosis of leishmaniasis in lagomorphs using a Bayesian approach.Veterinary medicine and science · 2016Article
- Article
- Development of loop-mediated isothermal amplification (LAMP) for simple detection of Leishmania infection.Parasites & vectors · 2015Article
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundLeishmania siamensis, a newly identified species, has been reported as a causative agent of leishmaniasis in Thailand. This organism has been identified and genetically characterized using PCR techniques based on several target genes. However, the sensitivities and specificities of these methods for the diagnosis of L. siamensis infection have never been evaluated.
methodsTo evaluate the sensitivities and specificities of PCR methods to detect L. siamensis infection, PCR for different genetic markers, i.e., the small subunit ribosomal RNA region (SSU-rRNA), the internal transcribed spacer 1 region (ITS1), cysteine protease B (cpb), cytochrome b (cyt b), heat shock protein 70 (hsp70), the spliced leader mini-exon, and the triose-phosphate isomerase (tim) genes were compared.
resultsBoth the ITS1-PCR and the SSU rRNA-PCR could detect promastigote of L. siamensis at concentrations as low as 0.05 parasites/μl or the DNA concentration at 2.3 pg/μl. Though the ITS1-PCR method only recognized 8 samples as positive, all of these could be assessed as true positive according to microscopic diagnosis and/or amplifying the results of the PCR and their sequencing, while other methods also produced false positive results. Compared with the ITS1-PCR method, the PCR amplified SSU-rRNA and cpb gene showed 100% sensitivity for the detection of L. siamensis in clinical specimens. The PCR amplified mini-exon and hsp70 gene also gave a high sensitivity of 87.5%. In contrast, the PCR methods for cyt b and tim gene showed low sensitivity. The PCR methods for cyt b, mini-exon and tim gene showed 100% specificity compared with the ITS1-PCR.
conclusionAs a result, the ITS1-PCR method is a suitable target for PCR-based detection of L. siamensis infection in clinical specimens due to its high sensitivity and specificity. The results of this study can be used for molecular diagnosis as well as in epidemiological studies of L. siamensis in affected areas.
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