ArticleCytometry. Part A : the journal of the International Society for Analytical Cytology2014
Transient partial permeabilization with saponin enables cellular barcoding prior to surface marker staining.
Article in Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed.
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- Immunophenotyping with (phospho)protein profiling and fluorescent cell barcoding for single-cell signaling analysis and biomarker discovery.NPJ precision oncology · 2024Review
- Approaching Mass Cytometry Translational Studies by Experimental and Data Curation Settings.Methods in molecular biology (Clifton, N.J.) · 2024Review
- Sample multiplexing in CyTOF: Path to optimize single-cell proteomic profiling.Cell signaling · 2024Article
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- Signature for Pain Recovery IN Teens (SPRINT): protocol for a multisite prospective signature study in chronic musculoskeletal pain.BMJ open · 2022Article
- Single-cell proteomics defines the cellular heterogeneity of localized prostate cancer.Cell reports. Medicine · 2022Article
- Mass Cytometry Assessment of Cell Phenotypes and Signaling States in Human Whole Blood.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Uncovering Pharmacological Opportunities for Cancer Stem Cells-A Systems Biology View.Frontiers in cell and developmental biology · 2022Review
- Mass Cytometry for the Characterization of Individual Cell Types in Ovarian Solid Tumors.Methods in molecular biology (Clifton, N.J.) · 2022Article
- CyTOFFrontiers in immunology · 2022Review
- Variation of Immune Cell Responses in Humans Reveals Sex-Specific Coordinated Signaling Across Cell Types.Frontiers in immunology · 2022Article
- Profound dysregulation of T cell homeostasis and function in patients with severe COVID-19.Allergy · 2021Article
- Multi-omics reveals clinically relevant proliferative drive associated with mTOR-MYC-OXPHOS activity in chronic lymphocytic leukemia.Nature cancer · 2021Article
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Authors and funding
8 authors.
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Abstract
Fluorescent cellular barcoding and mass-tag cellular barcoding are cytometric methods that enable high sample throughput, minimize inter-sample variation, and reduce reagent consumption. Previously employed barcoding protocols require that barcoding be performed after surface marker staining, complicating combining the technique with measurement of alcohol-sensitive surface epitopes. This report describes a method of barcoding fixed cells after a transient partial permeabilization with 0.02% saponin that results in efficient and consistent barcode staining with fluorescent or mass-tagged reagents while preserving surface marker staining. This approach simplifies barcoding protocols and allows direct comparison of surface marker staining of multiple samples without concern for variations in the antibody cocktail volume, antigen-antibody ratio, or machine sensitivity. Using this protocol, cellular barcoding can be used to reliably detect subtle differences in surface marker expression.
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