Evidence map›Paper›PMID 25273541›Full record

ArticleStem cells translational medicine2014

Engineering efficient retinal pigment epithelium differentiation from human pluripotent stem cells.

Amelia Lane, Lissa Rachel Philip, Ludmila Ruban, Kate Fynes, Matthew Smart, Amanda Carr, Chris Mason, Pete Coffey

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cells translational medicine, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

27 citing papers in PubMed, 1 synthesis or guideline pooled it.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Amelia LaneInstitute of Ophthalmology and Advanced Centre for Biochemical Engineering, University College London, London, United Kingdom amelia.lane@ucl.ac.uk.
Lissa Rachel PhilipInstitute of Ophthalmology and Advanced Centre for Biochemical Engineering, University College London, London, United Kingdom.
Ludmila RubanInstitute of Ophthalmology and Advanced Centre for Biochemical Engineering, University College London, London, United Kingdom.
Kate FynesInstitute of Ophthalmology and Advanced Centre for Biochemical Engineering, University College London, London, United Kingdom.
Matthew SmartInstitute of Ophthalmology and Advanced Centre for Biochemical Engineering, University College London, London, United Kingdom.
Amanda CarrInstitute of Ophthalmology and Advanced Centre for Biochemical Engineering, University College London, London, United Kingdom.
Chris MasonInstitute of Ophthalmology and Advanced Centre for Biochemical Engineering, University College London, London, United Kingdom.
Pete CoffeyInstitute of Ophthalmology and Advanced Centre for Biochemical Engineering, University College London, London, United Kingdom.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Human embryonic stem cells (hESCs) are a promising source of retinal pigment epithelium (RPE) cells: cells that can be used for the treatment of common and incurable forms of blindness, such as age-related macular degeneration. Although most hESC lines will produce a number of clusters of pigmented RPE cells within 30-50 days when allowed to spontaneously differentiate, the timing and efficiency of differentiation is highly variable. This could prove problematic in the design of robust processes for the large scale production of RPE cells for cell therapy. In this study we sought to identify, quantify, and reduce the sources of variability in hESC-RPE differentiation. By monitoring the emergence of pigmented cells over time, we show how the cell line, passaging method, passage number, and seeding density have a significant and reproducible effect on the RPE yield. To counter this variability, we describe the production of RPE cells from two cell lines in feeder-free, density controlled conditions using single cell dissociation and seeding that is more amenable to scaled up production. The efficacy of small molecules in directing differentiation toward the RPE lineage was tested in two hESC lines with divergent RPE differentiation capacities. Neural induction by treatment with a bone morphogenetic protein inhibitor, dorsomorphin, significantly enhanced the RPE yield in one cell line but significantly reduce it in another, generating instead a Chx10 positive neural progenitor phenotype. This result underlines the necessity to tailor differentiation protocols to suit the innate properties of different cell lines.

Indexed as

Cell DifferentiationNeural Stem CellsPluripotent Stem CellsRetinal Pigment EpitheliumCell Culture TechniquesCell LineHomeodomain ProteinsHumansTranscription FactorsHomeodomain ProteinsTranscription FactorsVSX2 protein, humanBioprocess engineeringCell differentiationCell therapyHuman embryonic stem cellsRetinal pigment epithelium

Identifiers

PMID25273541
PMCPMC4214845

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.