ArticleJournal of nanobiotechnology2014
Enhanced green fluorescent protein-mediated synthesis of biocompatible graphene.
Article in Journal of nanobiotechnology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Superchaotropic Stabilization of Monomeric Protein States.Biomacromolecules · 2026Article
- Towards greener reduced graphene oxide: a critical review of environmentally driven reduction strategies.RSC advances · 2026Review
- Nano Biomaterials in Drug Delivery and Tissue Engineering.Current drug delivery · 2026Review
- Development and comprehensive evaluation of scarless circularization systems for circular RNA therapeutics.Molecular therapy. Nucleic acids · 2025Article
- Further characterization and engineering of an 11-amino acid motif for enhancing recombinant soluble protein expression.Microbial cell factories · 2025Article
- A small-molecule carrier for the intracellular delivery of a membrane-impermeable protein with retained bioactivity.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Employing electrochemically derived pH gradients for Lab-on-PCB protein preconcentration devices.Microsystems & nanoengineering · 2024Article
- Graphene Oxide (GO) Materials-Applications and Toxicity on Living Organisms and Environment.Journal of functional biomaterials · 2022Review
- Evaluating the X-ray-Shielding Performance of Graphene-Oxide-Coated Nanocomposite Fabric.Materials (Basel, Switzerland) · 2022Article
- Fluorescent Protein Variants Generated by Reassembly between Skeleton and Chromophore.ACS omega · 2021Article
- Evaluation of Graphene Oxide Induced Cellular Toxicity and Transcriptome Analysis in Human Embryonic Kidney Cells.Nanomaterials (Basel, Switzerland) · 2019Article
- The effects of graphene and mesenchymal stem cells in cutaneous wound healing and their putative action mechanism.International journal of nanomedicine · 2019Review
- Effects of Reduced Graphene Oxides on Apoptosis and Cell Cycle of Glioblastoma Multiforme.International journal of molecular sciences · 2018Article
- Antibacterial Efficacy of Silver Nanoparticles on Endometritis Caused byInternational journal of molecular sciences · 2018Article
- Graphene Oxide-Silver Nanoparticles Nanocomposite Stimulates Differentiation in Human Neuroblastoma Cancer Cells (SH-SY5Y).International journal of molecular sciences · 2017Article
- Effects of Silver Nanoparticles on Multiple Drug-Resistant Strains of Staphylococcus aureus and Pseudomonas aeruginosa from Mastitis-Infected Goats: An Alternative Approach for Antimicrobial Therapy.International journal of molecular sciences · 2017Article
- Multifunctionalization of graphene and graphene oxide for controlled release and targeted delivery of anticancer drugs.American journal of translational research · 2017Review
- Quercetin-mediated synthesis of graphene oxide-silver nanoparticle nanocomposites: a suitable alternative nanotherapy for neuroblastoma.International journal of nanomedicine · 2017Article
- Silver Nanoparticle-Embedded Thin Silica-Coated Graphene Oxide as an SERS Substrate.Nanomaterials (Basel, Switzerland) · 2016Article
- Graphene oxide and titanium: synergistic effects on the biomineralization ability of osteoblast cultures.Journal of materials science. Materials in medicine · 2016Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundGraphene is the 2D form of carbon that exists as a single layer of atoms arranged in a honeycomb lattice and has attracted great interest in the last decade in view of its physical, chemical, electrical, elastic, thermal, and biocompatible properties. The objective of this study was to synthesize an environmentally friendly and simple methodology for the preparation of graphene using a recombinant enhanced green fluorescent protein (EGFP).
resultsThe successful reduction of GO to graphene was confirmed using UV-vis spectroscopy, and FT-IR. DLS and SEM were employed to demonstrate the particle size and surface morphology of GO and EGFP-rGO. The results from Raman spectroscopy suggest the removal of oxygen-containing functional groups from the surface of GO and formation of graphene with defects. The biocompatibility analysis of GO and EGFP-rGO in human embryonic kidney (HEK) 293 cells suggests that GO induces significant concentration-dependent cell toxicity in HEK cells, whereas graphene exerts no adverse effects on HEK cells even at a higher concentration (100 μg/mL).
conclusionsAltogether, our findings suggest that recombinant EGFP can be used as a reducing and stabilizing agent for the preparation of biocompatible graphene. The novelty and originality of this work is that it describes a safe, simple, and environmentally friendly method for the production of graphene using recombinant enhanced green fluorescent protein. Furthermore, the synthesized graphene shows excellent biocompatibility with HEK cells; therefore, biologically synthesized graphene can be used for biomedical applications. To the best of our knowledge, this is the first and novel report describing the synthesis of graphene using recombinant EGFP.
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