ArticleBiomechanics and modeling in mechanobiology2015
Cardiomyocyte subdomain contractility arising from microenvironmental stiffness and topography.
Article in Biomechanics and modeling in mechanobiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Biomimetic Cardiac Tissue Models for In Vitro Arrhythmia Studies.Biomimetics (Basel, Switzerland) · 2023Review
- Dynamic mechanobiology of cardiac cells and tissues: Current status and future perspective.Biophysics reviews · 2023Review
- Engineered microenvironment for the study of myofibroblast mechanobiology.Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society · 2021Article
- Transthyretin amyloid fibrils alter primary fibroblast structure, function, and inflammatory gene expression.American journal of physiology. Heart and circulatory physiology · 2021Article
- Review
- Cardiac tissue engineering therapeutic products to enhance myocardial contractility.Journal of muscle research and cell motility · 2020Review
- (De)form and Function: Measuring Cellular Forces with Deformable Materials and Deformable Structures.Advanced healthcare materials · 2020Review
- Stiffness Sensing by Cells.Physiological reviews · 2020Review
- Hang on tight: reprogramming the cell with microstructural cues.Biomedical microdevices · 2019Review
- PKC epsilon signaling effect on actin assembly is diminished in cardiomyocytes when challenged to additional work in a stiff microenvironment.Cytoskeleton (Hoboken, N.J.) · 2018Article
- Injectable hyaluronic acid based microrods provide local micromechanical and biochemical cues to attenuate cardiac fibrosis after myocardial infarction.Biomaterials · 2018Article
- Lipid signaling affects primary fibroblast collective migration and anchorage in response to stiffness and microtopography.Journal of cellular physiology · 2018Article
- Variation in stiffness regulates cardiac myocyte hypertrophy via signaling pathways.Canadian journal of physiology and pharmacology · 2016Article
- A myosin activator improves actin assembly and sarcomere function of human-induced pluripotent stem cell-derived cardiomyocytes with a troponin T point mutation.American journal of physiology. Heart and circulatory physiology · 2016Article
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Authors and funding
2 authors.
Funding
Abstract
Cellular structure and function are interdependent. To understand this relationship in beating heart cells, individual neonatal rat ventricular myocytes (NRVMs) were analyzed one and 3 days after plating when cultured on different stiffness (100, 400 kPa) and surface structures (flat or [Formula: see text] high, [Formula: see text] diameter, microposts spaced [Formula: see text] apart) manufactured from polydimethylsiloxane. Myofibril structure seen by immunohistochemistry was organized in three dimensions when NRVMs were attached to microposts. On day three, paxillin distribution near the post serving as cellular anchorage was quantified on both soft posts (12.04 % of total voxel count) and stiff posts (8.16 %). Living NRVMs were analyzed using line scans for sarcomeric shortening and shortening velocity, and traction force microscopy for surface stress and surface tension. One day after plating, NRVMs shortened more on soft posts ([Formula: see text] at [Formula: see text]) compared to either soft flat ([Formula: see text] at [Formula: see text]), stiff posts ([Formula: see text] at [Formula: see text]) or stiff flat ([Formula: see text] at [Formula: see text]). NRVMs have decreased shortening and shortening velocity on soft posts ([Formula: see text] at [Formula: see text]) compared to soft flat ([Formula: see text] at [Formula: see text]) substrates. The surface stress and surface tension increased over time for both soft post ([Formula: see text] and [Formula: see text] to [Formula: see text] and [Formula: see text]) and flat ([Formula: see text] and [Formula: see text] to [Formula: see text] and [Formula: see text]) substrates. Paxillin displacement during contraction on day three was significantly greater in NRVMs attached to soft posts [Formula: see text] compared to flat [Formula: see text] substrates. The volume and time creating four-dimensional data, interpreted by structural engineering theory, demonstrate subdomain structure is maintained by the counterbalance between the external load acting upon and the internal forces generated by the cardiomyocyte. These findings provide further insight into localized regulation of cellular mechanical function.
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