ArticleExperimental & applied acarology2015
RNAi-mediated knockdown of transformer-2 in the predatory mite Metaseiulus occidentalis via oral delivery of double-stranded RNA.
Article in Experimental & applied acarology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 23 citations in OpenAlex.
- Star Polycation Mediated dsRNA Improves the Efficiency of RNA Interference inNanomaterials (Basel, Switzerland) · 2022Article
- Molecular characterization, expression, and function of Vitellogenin genes in Phytoseiulus persimilis.Experimental & applied acarology · 2022Article
- Delivery of Double-Stranded RNAs (dsRNAs) Produced by Escherichia coli HT115(DE3) for Nontransgenic RNAi-Based Insect Pest Management.Methods in molecular biology (Clifton, N.J.) · 2022Article
- RNAi mediated knockdown of RpL11, RpS2, and tra-2 led to reduced reproduction of Phytoseiulus persimilis.Experimental & applied acarology · 2019Article
- Two of the three Transformer-2 genes are required for ovarian development in Aedes albopictus.Insect biochemistry and molecular biology · 2019Article
- Spermatophore producing process and sperm transfer in Phytoseiulus persimilis.Experimental & applied acarology · 2019Article
- Genome Sequencing of the Phytoseiid Predatory Mite Metaseiulus occidentalis Reveals Completely Atomized Hox Genes and Superdynamic Intron Evolution.Genome biology and evolution · 2016Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Little is known about the process of sex determination at the molecular level in Metaseiulus occidentalis, a parahaploid species and natural enemy of phytophagous pest mites. Detailed knowledge of the sex-determination pathway could allow genetic manipulation of M. occidentalis to produce more female offspring, which could improve its effectiveness as a biological control agent. RNA interference is useful for assessing the function of putative sex-determination genes by reducing or eliminating gene expression. In many insect species the transformer-2 (tra-2) gene is an upstream regulatory element in the sex-determination cascade, and knockdown of tra-2 expression can alter the sex ratio. We assessed whether oral delivery of tra-2 double-stranded RNA to M. occidentalis virgin females would affect the sex of her progeny. Females that ingested tra-2 dsRNA produced significantly fewer eggs compared to control females suggesting that tra-2 is somehow involved in reproduction by females. However, the sex ratio of the few progeny that were laid was not altered, so it is unclear whether tra-2 is involved in sex determination. This is an initial step towards elucidating the molecular components of sex determination in M. occidentalis.
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