ArticleThe Journal of clinical investigation1989
Receptor-mediated phagocytosis in human neutrophils is associated with increased formation of inositol phosphates and diacylglycerol. Elevation in cytosolic free calcium and formation of inositol phosphates can be dissociated from accumulation of diacylglycerol.
Article in The Journal of clinical investigation, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 83 citations in OpenAlex.
- Host-directed therapy for bacterial infections -Modulation of the phagolysosome pathway.Frontiers in immunology · 2023Review
- Targeting of protein kinase C-epsilon during Fcgamma receptor-dependent phagocytosis requires the epsilonC1B domain and phospholipase C-gamma1.Molecular biology of the cell · 2006Article
- Activated complement component 3 (C3) is required for ultraviolet induction of immunosuppression and antigenic tolerance.The Journal of experimental medicine · 1998Article
- Proximity oscillations of complement type 4 (alphaX beta2) and urokinase receptors on migrating neutrophils.Biophysical journal · 1997Article
- Article
- Activation of phospholipase D is tightly coupled to the phagocytosis of Mycobacterium tuberculosis or opsonized zymosan by human macrophages.The Journal of experimental medicine · 1996Article
- Activation of phospholipase D is an early event in integrin-mediated signalling leading to phagocytosis in human neutrophils.Inflammation · 1996Article
- Neutrophil-mediated tissue injury and its modulation.Intensive care medicine · 1995Review
- Activation of phospholipase C and phospholipase D by stimulation of adenosine A1, bradykinin or P2U receptors does not correlate well with protein kinase C activation.Naunyn-Schmiedeberg's archives of pharmacology · 1995Article
- Beta 2 integrin engagement triggers actin polymerization and phosphatidylinositol trisphosphate formation in non-adherent human neutrophils.The Journal of cell biology · 1993Article
- Enhancement of crystal induced neutrophil responses by opsonisation of calcium pyrophosphate dihydrate crystals.Annals of the rheumatic diseases · 1993Article
- Cytosolic free Ca2+ signals in single adherent human neutrophils: generation and functional role.European journal of pediatrics · 1993Review
- Cytosolic free calcium is essential for immunoglobulin G-stimulated intracellular killing of Staphylococcus aureus by human monocytes.Infection and immunity · 1992Article
- Impairment of neutrophil Fc gamma receptor mediated transmembrane signalling in active rheumatoid arthritis.Annals of the rheumatic diseases · 1992Article
- Pertussis toxin partially inhibits phagocytosis of immunoglobulin G-opsonized Staphylococcus aureus by human granulocytes but does not affect intracellular killing.Infection and immunity · 1992Article
- Roles of Ca2+ in human neutrophil responses to receptor agonists.The Biochemical journal · 1991Article
- G alpha 16, a G protein alpha subunit specifically expressed in hematopoietic cells.Proceedings of the National Academy of Sciences of the United States of America · 1991Article
- Modulation of phagocytic function in murine peritoneal macrophages by bombesin, gastrin-releasing peptide and neuromedin C.Immunology · 1991Article
- Tumor necrosis factor and CD11/CD18 (beta 2) integrins act synergistically to lower cAMP in human neutrophils.The Journal of cell biology · 1990Article
- Effects of chemotactic peptide f-Met-Leu-Phe (FMLP) on C3b receptor (CR1) expression and phagocytosis of microspheres by human neutrophils.Inflammation · 1990Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phagocytosis of C3bi- or IgG-opsonized yeast particles in human neutrophils was found to be associated with an increased formation of inositol phosphates and diacylglycerol. Pertussis toxin only marginally affected phagocytosis of IgG- and C3bi-opsonized particles and the associated formation of second messengers. Forskolin, which induced a threefold rise of cellular cAMP, however, markedly inhibited both C3bi- and IgG-mediated phagocytosis as well as the particle-induced formation of inositol phosphates and diacylglycerol. These observations are in contrast to what was found to occur with chemotactic factors and indicate that chemotactic and phagocytic signaling can be regulated independently in human neutrophils. Since C3bi-mediated phagocytosis has been shown to occur at vanishingly low cytosolic free calcium levels, calcium-depleted cells were used to study the importance of the inositol cycle for the engulfment of C3bi-opsonized particles. Despite a total lack of receptor-induced formation of inositol phosphates, a significantly increased accumulation of diacylglycerol accompanied the ingestion of C3bi-opsonized particles. These data show that the engulfment of C3bi-opsonized particles can occur independently of both a calcium transient and an increased inositol phosphate production. However, the observed accumulation of diacylglycerol, not derived from phosphoinositides, suggests that this second messenger play a role in the control of the engulfment process.
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