ArticleInternational journal of molecular sciences2014
Recombinant expression of a novel fungal immunomodulatory protein with human tumor cell antiproliferative activity from Nectria haematococca.
Article in International journal of molecular sciences, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 24 citations in OpenAlex.
- Fungal immunomodulatory proteins skew macrophages towards a pro-inflammatory and tumour inhibitory phenotype.Cancer immunology, immunotherapy : CII · 2026Article
- Characterization and anticancer bioactivity of the fungal immunomodulatory protein FIP-Gre from Ganoderma resinaceum.Molecular biology reports · 2026Article
- rFIP-nha activates macrophages towards a pro-inflammatory phenotype via AIM2 inflammasome modulation.Frontiers in cell and developmental biology · 2025Article
- Glycosylation Contributes to Thermostability and Proteolytic Resistance of rFIP-nha (Molecules (Basel, Switzerland) · 2023Article
- FIP-nha, a fungal immunomodulatory protein fromFood chemistry. Molecular sciences · 2022Article
- Proteomic Research on the Antitumor Properties of Medicinal Mushrooms.Molecules (Basel, Switzerland) · 2021Review
- Current Understanding of the Structure and Function of Fungal Immunomodulatory Proteins.Frontiers in nutrition · 2020Review
- RecombinantFrontiers in pharmacology · 2020Article
- Identification and Functional Characterization of a Novel Immunomodulatory Protein FromFrontiers in immunology · 2020Article
- Immunomodulatory Protein fromInternational journal of molecular sciences · 2019Article
- Identification of a Novel Anti-cancer Protein, FIP-bbo, from Botryobasidium botryosum and Protein Structure Analysis using Molecular Dynamic Simulation.Scientific reports · 2019Article
- Fungal Immunomodulatory Protein fromInternational journal of molecular sciences · 2018Article
- Characterisation of a New Fungal Immunomodulatory Protein from Tiger Milk mushroom, Lignosus rhinocerotis.Scientific reports · 2016Article
- Identification and Characterisation of a Novel Protein FIP-sch3 from Stachybotrys chartarum.PloS one · 2016Article
- Proteome exploration to provide a resource for the investigation of Ganoderma lucidum.PloS one · 2015Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To our best knowledge, all of the fungal immunomodulatory proteins (FIPs) have been successfully extracted and identified in Basidomycetes, with only the exception of FIP from ascomycete Nectria haematococca (FIP-nha) discovered through homology alignment most recently. In this work, a gene encoding FIP-nha was synthesized and recombinantly expressed in an Escherichia coli expression system. SDS-PAGE and MALDI-MS analyses of recombinant FIP-nha (rFIP-nha) indicated that the gene was successfully expressed. The yield of the bioactive FIP-nha protein was 42.7 mg/L. In vitro assays of biological activity indicated that the rFIP-nha caused hemagglutination of human and rabbit red blood cells, significantly stimulated mouse spleen lymphocyte proliferation, and enhanced expression of interleukin-2 (IL-2) released from mouse splenocytes, revealing a strong antitumor effect against HL60, HepG2 and MGC823. Through this work, we constructed a rapid and efficient method of FIP production, and suggested that FIP-nha is a valuable candidate for use in future medical care and pharmaceutical products.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.