ArticleeLife2014
Reconstitution of self-organizing protein gradients as spatial cues in cell-free systems.
Article in eLife, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
68 citing papers in PubMed.
- Devising a divisome for synthetic cells.Nature reviews. Chemistry · 2026Review
- Mimicking Cellular Membranes With Micropatterned Lipid Bilayers.Chemphyschem : a European journal of chemical physics and physical chemistry · 2026Article
- Cell-Size Confinement Drives Size-Dependent Scaling of Intracellular Reaction-Diffusion Waves for Robust Patterning.Small science · 2026Article
- Transition from alternating stripes to alternating labyrinths in oscillatory media.Physical review. E · 2025Article
- Programming scheduled self-assembly of circadian materials.Nature communications · 2025Article
- Robust and resource-optimal dynamic pattern formation of Min proteins in vivo.Nature physics · 2025Article
- Review
- Lipid Membrane Topographies Are Regulators for the Spatial Distribution of Liquid Protein Condensates.Nano letters · 2024Article
- Article
- Self-organization of mortal filaments and its role in bacterial division ring formation.Nature physics · 2024Article
- Artificial cell system as a tool for investigating pattern formation mechanisms of intracellular reaction-diffusion waves.Biophysics and physicobiology · 2024Review
- Forceful patterning: theoretical principles of mechanochemical pattern formation.EMBO reports · 2023Review
- The mechanism of MinD stability modulation by MinE in Min protein dynamics.PLoS computational biology · 2023Article
- Bacterial cell-size changes resulting from altering the relative expression of Min proteins.Nature communications · 2023Article
- Measuring flow-mediated protein drift across stationary supported lipid bilayers.Biophysical journal · 2023Article
- Actin network architecture can ensure robust centering or sensitive decentering of the centrosome.The EMBO journal · 2022Article
- In vitro assembly, positioning and contraction of a division ring in minimal cells.Nature communications · 2022Article
- Min waves without MinC can pattern FtsA-anchored FtsZ filaments on model membranes.Communications biology · 2022Article
- Mode selection mechanism in traveling and standing waves revealed by Min wave reconstituted in artificial cells.Science advances · 2022Article
- Catching a Walker in the Act-DNA Partitioning by ParA Family of Proteins.Frontiers in microbiology · 2022Review
8 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Intracellular protein gradients are significant determinants of spatial organization. However, little is known about how protein patterns are established, and how their positional information directs downstream processes. We have accomplished the reconstitution of a protein concentration gradient that directs the assembly of the cell division machinery in E.coli from the bottom-up. Reconstituting self-organized oscillations of MinCDE proteins in membrane-clad soft-polymer compartments, we demonstrate that distinct time-averaged protein concentration gradients are established. Our minimal system allows to study complex organizational principles, such as spatial control of division site placement by intracellular protein gradients, under simplified conditions. In particular, we demonstrate that FtsZ, which marks the cell division site in many bacteria, can be targeted to the middle of a cell-like compartment. Moreover, we show that compartment geometry plays a major role in Min gradient establishment, and provide evidence for a geometry-mediated mechanism to partition Min proteins during bacterial development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.