ArticleProceedings of the National Academy of Sciences of the United States of America2014
Solid-to-fluid-like DNA transition in viruses facilitates infection.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 30 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
30 citing papers in PubMed, 60 citations in OpenAlex.
- A Review on Recent Trends in Bacteriophages for Post-Harvest Food Decontamination.Microorganisms · 2025Review
- Temperature-dependent ejection evolution arising from active and passive effects in DNA viruses.Biophysical journal · 2024Article
- Nanomechanical resilience and thermal stability of RSJ2 phage.Scientific reports · 2024Article
- Article
- An Overview of Diverse Strategies To InactivateEcoSal Plus · 2023Review
- 'SAXS-osmometer' method provides measurement of DNA pressure in viral capsids and delivers an empirical equation of state.Nucleic acids research · 2023Article
- Temperature-induced DNA density transition in phage λ capsid revealed with contrast-matching SANS.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Role of DNA-DNA sliding friction and nonequilibrium dynamics in viral genome ejection and packaging.Nucleic acids research · 2023Article
- Geochemical constraints on bacteriophage infectivity in terrestrial environments.ISME communications · 2023Article
- AT-specific DNA visualization revisits the directionality of bacteriophage λ DNA ejection.Nucleic acids research · 2023Article
- Structural atlas of a human gut crassvirus.Nature · 2023Article
- The SARS-CoV-2 Hydra, a tiny monster from the 21st century: Thermodynamics of the BA.5.2 and BF.7 variants.Microbial risk analysis · 2023Article
- Review
- Nucleus size and its effect on nucleosome stability in living cells.Biophysical journal · 2022Article
- Thermoresponsive C22 phage stiffness modulates the phage infectivity.Scientific reports · 2022Article
- Predicting the capsid architecture of phages from metagenomic data.Computational and structural biotechnology journal · 2022Article
- C22 podovirus infectivity is associated with intermediate stiffness.Scientific reports · 2020Article
- Pressurized DNA state inside herpes capsids-A novel antiviral target.PLoS pathogens · 2020Article
- Pan-retroviral Nucleocapsid-Mediated Phase Separation Regulates Genomic RNA Positioning and Trafficking.Cell reports · 2020Article
- Studying viruses using solution X-ray scattering.Biophysical reviews · 2020Review
Corrections and comments
- Erratum issued
Authors and funding
9 authors at 5 institutions in 2 countries.
Funding
Abstract
Releasing the packaged viral DNA into the host cell is an essential process to initiate viral infection. In many double-stranded DNA bacterial viruses and herpesviruses, the tightly packaged genome is hexagonally ordered and stressed in the protein shell, called the capsid. DNA condensed in this state inside viral capsids has been shown to be trapped in a glassy state, with restricted molecular motion in vitro. This limited intracapsid DNA mobility is caused by the sliding friction between closely packaged DNA strands, as a result of the repulsive interactions between the negative charges on the DNA helices. It had been unclear how this rigid crystalline structure of the viral genome rapidly ejects from the capsid, reaching rates of 60,000 bp/s. Through a combination of single-molecule and bulk techniques, we determined how the structure and energy of the encapsidated DNA in phage λ regulates the mobility required for its ejection. Our data show that packaged λ-DNA undergoes a solid-to-fluid-like disordering transition as a function of temperature, resulting locally in less densely packed DNA, reducing DNA-DNA repulsions. This process leads to a significant increase in genome mobility or fluidity, which facilitates genome release at temperatures close to that of viral infection (37 °C), suggesting a remarkable physical adaptation of bacterial viruses to the environment of Escherichia coli cells in a human host.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.