ArticleJournal of proteome research2014
In-depth quantitative proteomic analysis of de novo protein synthesis induced by brain-derived neurotrophic factor.
Article in Journal of proteome research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 53 citations in OpenAlex.
- Bioorthogonal Chemistry in Neurobiology: From Imaging to Proteomics.Chembiochem : a European journal of chemical biology · 2026Review
- Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) for Quantitative Proteomics.Advances in experimental medicine and biology · 2026Review
- Organ-Specific Small Protein Networks in 100 kDa Ultrafiltrates: Functional Analysis and Implications for Neuroregenerative Medicine.International journal of molecular sciences · 2025Article
- Acid Cleavable Biotin-Alkyne Improves Sensitivity for Direct Detection of Biotin Labeled Peptides in Biorthogonal Noncanonical Amino Acid Tagging Analysis.Journal of proteome research · 2024Article
- Acid cleavable biotin-alkyne improves sensitivity for direct detection of biotin labeled peptides in BONCAT analysis.bioRxiv : the preprint server for biology · 2024Article
- Tracing active members in microbial communities by BONCAT and click chemistry-based enrichment of newly synthesized proteins.ISME communications · 2024Article
- An integrated workflow for quantitative analysis of the newly synthesized proteome.Nature communications · 2023Article
- Stable Isotope Labeling by Amino Acids and Bioorthogonal Noncanonical Amino Acid Tagging in Cultured Primary Neurons.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Advances in stable isotope labeling: dynamic labeling for spatial and temporal proteomic analysis.Molecular omics · 2022Review
- Recent advancements in mass spectrometry-based tools to investigate newly synthesized proteins.Current opinion in chemical biology · 2022Review
- A new strategy to uncover fragile X proteomic biomarkers using the nascent proteome of peripheral blood mononuclear cells (PBMCs).Scientific reports · 2021Article
- Age-dependent shift in the de novo proteome accompanies pathogenesis in an Alzheimer's disease mouse model.Communications biology · 2021Article
- LKB1-MARK2 signalling mediates lipopolysaccharide-induced production of cytokines in mouse macrophages.Journal of cellular and molecular medicine · 2020Article
- Emerging proteomic approaches to identify the underlying pathophysiology of neurodevelopmental and neurodegenerative disorders.Molecular autism · 2020Review
- Proteomic Techniques to Examine Neuronal Translational Dynamics.International journal of molecular sciences · 2019Review
- Quantitative proteomics identifies proteins that resist translational repression and become dysregulated in ALS-FUS.Human molecular genetics · 2019Article
- Prediction of seed gene function in progressive diabetic neuropathy by a network-based inference method.Experimental and therapeutic medicine · 2019Article
- Altered steady state and activity-dependent de novo protein expression in fragile X syndrome.Nature communications · 2019Article
- Quantitative Comparison of Proteomes Using SILAC.Current protocols in protein science · 2019Review
- Translatomics: The Global View of Translation.International journal of molecular sciences · 2019Review
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Measuring the synthesis of new proteins in the context of a much greater number of pre-existing proteins can be difficult. To overcome this obstacle, bioorthogonal noncanonical amino acid tagging (BONCAT) can be combined with stable isotope labeling by amino acid in cell culture (SILAC) for comparative proteomic analysis of de novo protein synthesis (BONLAC). In the present study, we show that alkyne resin-based isolation of l-azidohomoalanine (AHA)-labeled proteins using azide/alkyne cycloaddition minimizes contamination from pre-existing proteins. Using this approach, we isolated and identified 7414 BONCAT-labeled proteins. The nascent proteome isolated by BONCAT was very similar to the steady-state proteome, although transcription factors were highly enriched by BONCAT. About 30% of the methionine residues were replaced by AHA in our BONCAT samples, which allowed for identification of methionine-containing peptides. There was no bias against low-methionine proteins by BONCAT at the proteome level. When we applied the BONLAC approach to screen for brain-derived neurotrophic factor (BDNF)-induced protein synthesis, 53 proteins were found to be significantly changed 2 h after BDNF stimulation. Our study demonstrated that the newly synthesized proteome, even after a short period of stimulation, can be efficiently isolated by BONCAT and analyzed to a depth that is similar to that of the steady-state proteome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.