ArticleChemical senses2014
A determinant of odorant specificity is located at the extracellular loop 2-transmembrane domain 4 interface of an Anopheles gambiae odorant receptor subunit.
Article in Chemical senses, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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26 citing papers in PubMed, 49 citations in OpenAlex.
- Molecular bases of insect odorant receptor function: specificity and evolution.Biological reviews of the Cambridge Philosophical Society · 2026Review
- Odorant ReceptorInsects · 2025Article
- Life-stage dependent behavior mimics chemosensory repertoire diversity in a belowground, specialist herbivore.G3 (Bethesda, Md.) · 2025Article
- Template-based modeling of insect odorant receptors outperforms AlphaFold3 for ligand binding predictions.Scientific reports · 2024Article
- Evolutionary shifts in pheromone receptors contribute to speciation in four Helicoverpa species.Cellular and molecular life sciences : CMLS · 2023Article
- A tale of two copies: Evolutionary trajectories of moth pheromone receptors.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Two odorant receptors regulate 1-octen-3-ol induced oviposition behavior in the oriental fruit fly.Communications biology · 2023Article
- Heterologous investigation of metabotropic and ionotropic odorant receptors in ab3A neurons ofFrontiers in molecular biosciences · 2023Article
- Single amino acid residue mediates reciprocal specificity in two mosquito odorant receptors.eLife · 2022Article
- Protocol to identify ligands of odorant receptors using two-electrode voltage clamp combined with theSTAR protocols · 2022Article
- Review
- A single point mutation causes one-way alteration of pheromone receptor function in twoiScience · 2021Article
- Characterization ofInsects · 2021Article
- Transcriptional variation of sensory-related genes in natural populations of Aedes albopictus.BMC genomics · 2020Article
- Sequence-Based Prediction of Olfactory Receptor Responses.Chemical senses · 2019Article
- Molecular and Functional Evolution at the Odorant Receptor Or22 Locus in Drosophila melanogaster.Molecular biology and evolution · 2019Article
- A Functional Agonist of Insect Olfactory Receptors: Behavior, Physiology and Structure.Frontiers in cellular neuroscience · 2019Article
- Article
- Odorant Receptors and Odorant-Binding Proteins as Insect Pest Control Targets: A Comparative Analysis.Frontiers in physiology · 2018Review
- Mutant cycle analysis identifies a ligand interaction site in an odorant receptor of the malaria vectorThe Journal of biological chemistry · 2017Article
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4 authors at 3 institutions in 2 countries.
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Abstract
To explore the structural basis for odorant specificity in odorant receptors of the human malaria vector mosquito, Anopheles gambiae, odorant-binding subunits (Agam\Ors) expressed in Xenopus oocytes in combination with Agam\Orco (coreceptor subunit) were assayed by 2-electrode voltage clamp against 25 structurally related odorants. Agam\Or13 and Agam\Or15 display 82% amino acid identity and had similar, but somewhat distinct odorant response profiles. The ratio of acetophenone to 4-methylphenol responses was used in a mutation-based analysis of Agam\Or15, interchanging 37 disparate residues between Agam\Or15 and Agam\Or13. Eleven mutations caused significant changes in odorant responsiveness. Mutation of alanine 195 resulted in the largest shift in response ratio from Agam\Or15 toward Agam\Or13. Concentration-response analysis for a series of mutations of residue 195 revealed a large effect on acetophenone sensitivity, with EC50 values varying by >1800-fold and correlating with residue side chain length. Similar results were obtained for propiophenone and benzaldehyde. But, for other odorants, such as 4-methylphenol, 4-methylbenzaldehyde, and 4-methylpropiophenone, the effect of mutation was much smaller (EC50 values varied by ≤16-fold). These results show that alanine 195, putatively located at the second extracellular loop/fourth transmembrane domain interface, plays a critical role in determining the odorant response specificity of Agam\Or15.
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