ArticleWorld journal of microbiology & biotechnology2014
Recombinant expression of glpK and glpD genes improves the accumulation of shikimic acid in E. coli grown on glycerol.
Article in World journal of microbiology & biotechnology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 19 citations in OpenAlex.
- Current Trends in Acetins Production: Green versus Non-Green Synthesis.Molecules (Basel, Switzerland) · 2022Review
- Genome Insights of the Plant-Growth Promoting BacteriumFrontiers in microbiology · 2020Article
- High-yield production of L-serine from glycerol by engineered Escherichia coli.Journal of industrial microbiology & biotechnology · 2019Article
- Novel technologies combined with traditional metabolic engineering strategies facilitate the construction of shikimate-producing Escherichia coli.Microbial cell factories · 2017Review
- Improving methionine and ATP availability by MET6 and SAM2 co-expression combined with sodium citrate feeding enhanced SAM accumulation in Saccharomyces cerevisiae.World journal of microbiology & biotechnology · 2016Article
- Metabolic engineering of Mortierella alpina for arachidonic acid production with glycerol as carbon source.Microbial cell factories · 2015Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Shikimic acid (SA) is an industrially important chiral compound used in diverse commercial applications, and the insufficient supply by isolation from plants and expensive chemical synthesis of SA has increased the importance of developing strategies for SA synthesis. In our previous studies, glycerol was observed to be an effective carbon source for SA accumulation in E. coli DHPYAAS-T7, where the PTS operon (ptsHIcrr) and aroL and aroK genes were inactivated, and the tktA, glk, aroE, aroF (fbr) , and aroB genes were overexpressed. For further investigation of the effects of glycerol aerobic fermentation on SA accumulation in E. coli BL21(DE3), the glpD, glpK genes and tktA, glk, aroE, aroF (fbr) , aroB genes were overexpressed simultaneously. The results indicated that SA production was increased 5.6-fold, while the yield was increased 5.3-fold over that of parental strain in shake flasks. It is demonstrated that the aerobic fermentation of glycerol associated with glpD and glpK gene overexpression increased glycerol flux, resulting in higher SA accumulation in E. coli BL21(DE3)-P-DK.
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